Characterization of self-assembled bioflocculant from the microbial consortium and its applications.

Bioflocculant has been recently exploited in various research activities. In this research, we report for the first time that a novel bioflocculant can self-assemble into nanoparticles with an irregular structure in solution. Bioflocculant has been developed from novel consortium encompassing Enterococcus faecalis, Proteus mirabilis, Lysini bacillus sp., inoculated into the modified medium such as hydrolyzed wheat bran and rice bran extract. Characterization of bioflocculant shows that it is made up of mannose, fructose, raffinose, and galactose with a slightly negative charges. They are further characterized by FTIR and XPS. 3D-EEM and MALDITOF-MS are confirmed the proteoglycan nature of the bioflocculant. These bioflocculant not only exhibits greater biosorption of heavy metals by self-flocculating (or) self-aggregating activity, but also possesses mosquitocidal and anti-biofilm activity due to its cell surface modification. Further research have to be carried out regarding the mechanism of self-assembly nature of bioflocculant into nanoparticle in solution which provide a new path for bioremediation and biomedical application.