Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, India.
Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, India.
J Environ Manage. 2020 Mar 15;258:110000. doi: 10.1016/j.jenvman.2019.110000. Epub 2020 Jan 7.
Bioflocculant has been recently exploited in various research activities. In this research, we report for the first time that a novel bioflocculant can self-assemble into nanoparticles with an irregular structure in solution. Bioflocculant has been developed from novel consortium encompassing Enterococcus faecalis, Proteus mirabilis, Lysini bacillus sp., inoculated into the modified medium such as hydrolyzed wheat bran and rice bran extract. Characterization of bioflocculant shows that it is made up of mannose, fructose, raffinose, and galactose with a slightly negative charges. They are further characterized by FTIR and XPS. 3D-EEM and MALDITOF-MS are confirmed the proteoglycan nature of the bioflocculant. These bioflocculant not only exhibits greater biosorption of heavy metals by self-flocculating (or) self-aggregating activity, but also possesses mosquitocidal and anti-biofilm activity due to its cell surface modification. Further research have to be carried out regarding the mechanism of self-assembly nature of bioflocculant into nanoparticle in solution which provide a new path for bioremediation and biomedical application.
生物絮凝剂最近在各种研究活动中得到了广泛应用。在这项研究中，我们首次报道了一种新型生物絮凝剂可以在溶液中自组装成具有不规则结构的纳米颗粒。生物絮凝剂是由新型共生菌群（包括粪肠球菌、奇异变形杆菌和解淀粉芽孢杆菌）接种到改良培养基（如水解麦麸和米糠提取物）中开发的。生物絮凝剂的特性表明，它由甘露糖、果糖、棉子糖和半乳糖组成，带轻微负电荷。它们进一步通过傅里叶变换红外光谱（FTIR）和 X 射线光电子能谱（XPS）进行了表征。3D-EEM 和 MALDITOF-MS 证实了生物絮凝剂的蛋白聚糖性质。这些生物絮凝剂不仅通过自絮凝（或）自聚集活性表现出对重金属更强的生物吸附能力，而且由于其细胞表面修饰还具有杀蚊和抗生物膜活性。需要进一步研究生物絮凝剂在溶液中自组装成纳米颗粒的内在机制，这为生物修复和生物医学应用提供了新的途径。
