State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China; Department of Marine Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, India.
Environ Res. 2021 Sep;200:111708. doi: 10.1016/j.envres.2021.111708. Epub 2021 Jul 16.
The removal of toxic heavy metal ions from contaminated environments is a great challenge and requires an alternative rapid, efficient, economical bioremediation approach. Henceforth, bioflocculant producing endophytic actinobacterial sp. was isolated from heavy metal contaminated marine environments for heavy metal biosorption process. After molecular characterization, the isolated actinomycete starin was Nocardiopsis sp. GRG 3 (KT235642). It was indicated that the maximum flocculating activity of 80.90% with glucose, and yield is 4.52 g L. The optimum flocculating activity was reached at pH 7 in the presence of CaCl ions. Further, the bioflocculent produced Nocardiopsis sp. GRG 3 (KT235642) was characterized by fourier transform infrared analysis spectra (FTIR) and displayed the presence of carboxyl, hydroxyl, amino groups and characteristic of more polysaccharide and protein. The heavy metal sorption by bioflocculant Nocardiopsis sp. GRG 3 (KT235642) was effectively removed 55.90% Cd, 85.90% Cr, 74.7% Pb, and 51.90% Hg. Therefore, this study was proved that the bioflocculant derived from endophytic actinobacteria, Nocardiopsis sp. GRG 3 (KT235642) as a effective alternative method for decreasing the heavy metals towards sustainable environmental management.
从受污染的环境中去除有毒重金属离子是一项巨大的挑战，需要一种替代的快速、高效、经济的生物修复方法。因此，从重金属污染的海洋环境中分离出了产生物絮凝剂的内生放线菌菌株，用于重金属生物吸附过程。经过分子鉴定，分离出的放线菌菌株为 Nocardiopsis sp. GRG 3 (KT235642)。结果表明，该菌株在葡萄糖存在下的最大絮凝活性为 80.90%，产率为 4.52 g/L。在 pH 7 和 CaCl2 离子存在下，达到最佳絮凝活性。此外，由 Nocardiopsis sp. GRG 3 (KT235642)产生的生物絮凝剂通过傅里叶变换红外分析光谱 (FTIR) 进行了表征，并显示存在羧基、羟基、氨基，且具有更多多糖和蛋白质的特征。生物絮凝剂 Nocardiopsis sp. GRG 3 (KT235642) 对重金属的吸附能有效去除 55.90%的 Cd、85.90%的 Cr、74.7%的 Pb 和 51.90%的 Hg。因此，这项研究证明了源自内生放线菌 Nocardiopsis sp. GRG 3 (KT235642)的生物絮凝剂作为一种有效的替代方法，可用于减少重金属，实现可持续的环境管理。
