不同加工方法对叶提取物中植物化学成分含量和神经保护活性的影响。
Effect of Different Processing Methods on Phytochemical Contents and Neuroprotective Activity of Leaves Extract.
机构信息
Department of Central Laboratory, Affiliated Hospital of Jining Medical University, Jining 272000, China.
School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
出版信息
Biomed Res Int. 2019 Dec 23;2019:1717090. doi: 10.1155/2019/1717090. eCollection 2019.
is a new food source and traditional folk medicine in China. Previous studies have demonstrated the antidepressant activity of extract by both and experiments. The effects of different pretreatments on phytochemical contents and neuroprotective activity of extract were further investigated in order to develop an optimal processing method that makes the extraction more efficient. Six different powders of leaves were prepared by different pretreatments. The particle size and morphology were examined by using a Malvern particle size analyzer and scanning electron microscopy, respectively. The results showed that the percentage of powder particle size within a range of 0.2∼40 m was up to 79.18% after press-shear assisted interaction technology pretreatment by 2% addition of shellfish shell powder, and the cells were broken completely. Additionally, the contents of flavonoids, polysaccharides, polyphenols, saponins, and catechin in the extract were 11.78 ± 0.62%, 34.60 ± 3.37%, 6.15 ± 0.29%, 9.43 ± 1.19%, and 1.99 ± 0.11%, respectively, which were higher than those of the other five extracts. Moreover, the extract had the strongest neuroprotective activity by comparing the neuroprotective effect of different extracts on corticosterone-induced neurotoxicity in differentiated PC12 cells. It is concluded that press-shear assisted interaction technology with 2% addition of shellfish shell powder pretreatment, to a great extent, improved the dissolution of bioactive ingredients in .
是中国的一种新的食物来源和传统民间药物。先前的研究已经证明了 提取物的抗抑郁活性，通过 和 实验。为了开发一种更有效的提取方法，进一步研究了不同预处理方法对 提取物中植物化学成分含量和神经保护活性的影响。用不同的预处理方法制备了 叶的六种不同粉末。分别使用马尔文粒度分析仪和扫描电子显微镜检查了粒径和形态。结果表明，在添加 2%贝壳粉的压剪辅助相互作用技术预处理后，粒径在 0.2∼40μm范围内的粉末百分比达到 79.18%，细胞完全破裂。此外，提取物中类黄酮、多糖、多酚、皂苷和儿茶素的含量分别为 11.78±0.62%、34.60±3.37%、6.15±0.29%、9.43±1.19%和 1.99±0.11%，高于其他五种提取物。此外，通过比较不同提取物对皮质酮诱导的分化 PC12 细胞神经毒性的神经保护作用，发现提取物具有最强的神经保护活性。结论：压剪辅助相互作用技术添加 2%贝壳粉预处理，在很大程度上提高了 中生物活性成分的溶解。
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