School of Life Science, Shandong University, Jinan 250100, PR China.
National Glycoengineering Research Center, Shandong University, Jinan 250100, PR China.
Int J Biol Macromol. 2018 Aug;115:718-726. doi: 10.1016/j.ijbiomac.2018.04.128. Epub 2018 Apr 25.
An extracellular polysaccharide (EPS1-1) extracted from the fermentation broth of Rhizopus nigricans has been proved to enhance the immunity of immunosuppressed mice. The purpose of this study was to investigate the beneficial effects of EPS1-1 on the intestinal immunity of mice with colorectal cancer induced by azoxymethane (AOM) and dextran sodium sulfate (DSS). The results showed that EPS1-1 could resist hydrolysis in an artificial stomach. Oral EPS1-1 modulated gut microbiota and increased the concentration of total short-chain fatty acids (SCFAs) in the feces of colorectal cancer mice compared with the AOM/DSS only-treated mice. Furthermore, EPS1-1 increased the villus length, ratio of villus length and crypt depth in colonic tissues, and improved the number of acid mucus-secreting goblet cells in mice with colorectal cancer. These findings suggest that EPS1-1 might play an important role in the improvement of intestinal function in mice with colorectal cancer, which indicate its strong potential as efficient bio-secure immunotherapy for clinical applications or adjuvant drug.
从黑根霉发酵液中提取的胞外多糖（EPS1-1）已被证明能增强免疫抑制小鼠的免疫力。本研究旨在探讨 EPS1-1 对氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）诱导的结直肠癌小鼠肠道免疫的有益作用。结果表明，EPS1-1 能抵抗人工胃液的水解。与仅用 AOM/DSS 处理的小鼠相比，口服 EPS1-1 可调节肠道微生物群，并增加结直肠癌小鼠粪便中总短链脂肪酸（SCFA）的浓度。此外，EPS1-1 增加了结直肠组织中绒毛长度、绒毛长度与隐窝深度的比值，并改善了结直肠癌小鼠中酸性黏液分泌杯状细胞的数量。这些发现表明，EPS1-1 可能在改善结直肠癌小鼠的肠道功能方面发挥重要作用，这表明其作为临床应用或辅助药物的有效生物安全免疫疗法具有巨大潜力。
