基于酪氨酸的化学传感器在水溶液和活细胞中对汞(II)进行高灵敏度的开启型检测。
Highly sensitive turn-on detection of mercury(II) in aqueous solutions and live cells with a chemosensor based on tyrosine.
机构信息
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637009, PR China.
Department of Urology, Institute of Urology, Gansu Nephro-Urological Clinical Center, Key Laboratory of Urological Diseases in Gansu Province, The Second Hospital of Lanzhou University, Lanzhou, Gansu 730030, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Apr 5;230:118004. doi: 10.1016/j.saa.2019.118004. Epub 2020 Jan 7.
Herein, we reported a novel fluorescent chemosensor (DY) based on dansyl group and tyrosine by solid phase peptide synthesis (SPPS) for the detection of mercury(II) ions with excellent selectivity among 17 different metal ions. As designed, DY exhibited a sensitive fluorescence "turn-on" response to Hg with low detection limits of 22.65 nM. A stoichiometric ratio (2: 1) of chemosensor DY and Hg ions was determined by a Job's plot, fluorescent titration and the ESI-MS spectra. Especially, the reversible of DY-Hg with EDTA establishes the reuse of DY, and the circulation effect was very good. Furthermore, the wide pH range of 6-10 makes it capable of application in biological systems. Moreover, DY has been successfully applied to the detection of Hg ions and EDTA in living HeLa and HK2 cells based on low cytotoxicity and good membrane permeability.
在此，我们通过固相多肽合成（SPPS）报道了一种基于丹磺酰基和酪氨酸的新型荧光化学传感器（DY），可在 17 种不同金属离子中对汞（II）离子进行出色的选择性检测。如设计的那样，DY 对 Hg 表现出灵敏的荧光“开启”响应，检测限低至 22.65 nM。通过 Job 图、荧光滴定和 ESI-MS 光谱确定了化学传感器 DY 和 Hg 离子的化学计量比（2:1）。特别是，DY-Hg 与 EDTA 的可逆性建立了 DY 的可重复使用性，并且循环效果非常好。此外，6-10 的宽 pH 范围使其能够应用于生物系统。此外，DY 已成功应用于基于低细胞毒性和良好膜通透性的活 HeLa 和 HK2 细胞中 Hg 离子和 EDTA 的检测。
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