Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637002, PR China.
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637002, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:735-742. doi: 10.1016/j.saa.2018.07.001. Epub 2018 Jul 5.
The zinc ions (Zn) play extremely irreplaceable role in the organism and the environment, the design and synthesis of a biomolecule-based fluorescence chemosensor for the detection of Zn with high sensitivity is very important. Herein, a novel tetrapeptide-based dansyl fluorescent "turn-on" response chemosensor (L) has been designed and synthesized by solid phase peptide synthesis (SPPS). As designed, L can detect Zn ions with specifically and sensitively based on photo-induced electron transfer (PET) mechanism in 100% aqueous solutions, and other metal ions do not interfere with Zn ions recognition. The stoichiometric ratio of L with Zn ions was 2:1, which matches with fluorescence titration and Job-plot assay. In addition, the reversibility and circularly process of the detection of L was confirmed by adding bonding agent NaEDTA. Moreover, L exhibits excellent biocompatibility and low biotoxicity with the limit of detection (LOD) for Zn about 18 nM, and has been successfully utilized for fluorescence imaging of Zn ions in living HeLa cells under physiological conditions.
锌离子（Zn）在生物体和环境中起着极其不可替代的作用，设计和合成基于生物分子的荧光化学传感器用于高灵敏度检测 Zn 非常重要。本文通过固相肽合成（SPPS）设计和合成了一种新型的基于丹磺酰基荧光“开启”响应的四肽荧光化学传感器（L）。设计上，L 可以基于光诱导电子转移（PET）机制在 100%水溶液中特异性和灵敏地检测 Zn 离子，而其他金属离子不会干扰 Zn 离子的识别。L 与 Zn 离子的化学计量比为 2:1，与荧光滴定和 Job 图测定相吻合。此外，通过加入结合剂 NaEDTA 证实了检测 L 的可逆性和循环过程。此外，L 表现出良好的生物相容性和低细胞毒性，其对 Zn 的检测限（LOD）约为 18 nM，并已成功用于生理条件下活 HeLa 细胞中 Zn 离子的荧光成像。
