Department of Chemistry, University of Calcutta, 92, A. P. C. Road, Kolkata, 700 009, India.
Protein Folding and Dynamics Laboratory, Structural Biology and Bio-informatics Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata, 700032, India.
Anal Bioanal Chem. 2019 Feb;411(6):1143-1157. doi: 10.1007/s00216-018-1546-0. Epub 2019 Jan 9.
A novel rhodamine-tryptamine conjugate-based fluorescent and chromogenic chemosensor (RTS) for detection of Hg present in water was reported. After gradual addition of Hg in aqueous methanol solution of RTS, a strong orange fluorescence and deep-pink coloration were observed. The probe showed high selectivity towards Hg compared to other competitive metal ions. The 1:1 binding stoichiometry between RTS and Hg was established by Job's plot analysis and mass spectroscopy. Initial studies showed that the synthesized probe RTS possessed fair non-toxicity and effectively passed through cell walls of model cell systems, viz., human neuroblastoma (SHSY5Y) cells and cervical cells (HeLa) to detect intercellular Hg ions, signifying its utility in biological system. The limit of detection (LOD) was found to be 2.1 nM or 0.42 ppb by fluorescence titration. Additionally, the potential relevance of synthesized chemosensor for detecting Hg ions in environmental water samples has been demonstrated. Graphical abstract ᅟ.
一种基于新型罗丹明-色胺偶联物的荧光比色化学传感器（RTS）被报道用于检测水中的 Hg。在 RTS 的水甲醇溶液中逐渐加入 Hg 后，观察到强橙色荧光和深粉色显色。与其他竞争金属离子相比，探针对 Hg 表现出高选择性。通过 Job 图分析和质谱确定了 RTS 与 Hg 之间 1:1 的结合计量关系。初步研究表明，合成探针 RTS 具有良好的非毒性，并能有效地穿透模型细胞系统（即人神经母细胞瘤（SHSY5Y）细胞和宫颈细胞（HeLa）的细胞壁，以检测细胞间 Hg 离子，表明其在生物系统中的应用。通过荧光滴定法发现检测限（LOD）为 2.1 nM 或 0.42 ppb。此外，还证明了合成化学传感器在检测环境水样中 Hg 离子方面的潜在相关性。
