Ase K, Saito N, Shearman M S, Kikkawa U, Ono Y, Igarashi K, Tanaka C, Nishizuka Y
Department of Biochemistry, Kobe University School of Medicine, Japan.
J Neurosci. 1988 Oct;8(10):3850-6. doi: 10.1523/JNEUROSCI.08-10-03850.1988.
Immunohistochemical and biochemical studies with subspecies-specific antibodies have revealed that beta I- and beta II-subspecies of protein kinase C, which result from alternative splicing of a single RNA transcript, show different regional expression in rat CNS. In the cerebellar cortex, beta I-subspecies is localized mainly in the granular layer, whereas beta II-subspecies is found predominantly in the molecular layer, most apparently in the presynaptic nerve endings that terminate at Purkinje cells. These distribution patterns are in sharp contrast to that of gamma-subspecies, which is most abundant within the Purkinje cells. The different patterns of expression imply that the multiple subspecies of protein kinase C may each have a specific function in modulating the neuronal activity of particular cell types.
使用亚种特异性抗体进行的免疫组织化学和生化研究表明，蛋白激酶C的βI和βII亚种由单一RNA转录本的可变剪接产生，在大鼠中枢神经系统中表现出不同的区域表达。在小脑皮质中，βI亚种主要定位于颗粒层，而βII亚种主要存在于分子层，最明显的是在终止于浦肯野细胞的突触前神经末梢中。这些分布模式与γ亚种形成鲜明对比，γ亚种在浦肯野细胞中最为丰富。不同的表达模式意味着蛋白激酶C的多个亚种可能各自在调节特定细胞类型的神经元活动中具有特定功能。
