Kosaka Y, Ogita K, Ase K, Nomura H, Kikkawa U, Nishizuka Y
Department of Biochemistry, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 1988 Mar 30;151(3):973-81. doi: 10.1016/s0006-291x(88)80461-3.
Expression of multiple subspecies of protein kinase C (PKC) was studied in various rat tissues. Three types of the enzyme designated type I, II, and III were analyzed, which have the structures of gamma-, beta- (beta I- and beta II-), and alpha-sequence, respectively. Type I enzyme was found only in the central nervous tissue, whereas type III enzyme appeared to be commonly present in various tissues such as liver, spleen, lung, testis, heart, and kidney. Type II enzyme was also found in these tissues. However, immunoblot and biochemical analysis indicated that type II enzyme of lung and heart was distinct from that of other tissues. The tissue-specific expression of PKC suggests that each subspecies of this enzyme has a defined function in processing and modulating tissue responses to external stimuli.
在各种大鼠组织中研究了蛋白激酶C（PKC）多种亚型的表达。分析了三种类型的该酶，分别命名为I型、II型和III型，它们分别具有γ-、β-（βI和βII-）和α-序列结构。I型酶仅在中枢神经组织中发现，而III型酶似乎普遍存在于各种组织中，如肝脏、脾脏、肺、睾丸、心脏和肾脏。II型酶也在这些组织中发现。然而，免疫印迹和生化分析表明，肺和心脏的II型酶与其他组织的不同。PKC的组织特异性表达表明，该酶的每个亚型在处理和调节组织对外界刺激的反应中具有特定功能。
