针对肠道病毒蛋白酶的抗体反应可能是急性感染的标志物。

Antibody Responses against Enterovirus Proteases are Potential Markers for an Acute Infection.

机构信息

Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.

Karolinska Institutet, The Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska University Hospital, 14152 Stockholm, Sweden.

出版信息

Viruses. 2020 Jan 9;12(1):78. doi: 10.3390/v12010078.

BACKGROUND

Enteroviruses are a group of common non-enveloped RNA viruses that cause symptoms ranging from mild respiratory infections to paralysis. Due to the abundance of enterovirus infections it is hard to distinguish between on-going and previous infections using immunological assays unless the IgM fraction is studied.

METHODS

In this study we show using Indirect ELISA and capture IgM ELISA that an IgG antibody response against the nonstructural enteroviral proteins 2A and 3C can be used to distinguish between IgM positive ( = 22) and IgM negative ( = 20) human patients with 83% accuracy and a diagnostic odds ratio of 30. Using a mouse model, we establish that the antibody response to the proteases is short-lived compared to the antibody response to the structural proteins in. As such, the protease antibody response serves as a potential marker for an acute infection.

CONCLUSIONS

Antibody responses against enterovirus proteases are shorter-lived than against structural proteins and can differentiate between IgM positive and negative patients, and therefore they are a potential marker for acute infections.

背景

肠道病毒是一组常见的无包膜 RNA 病毒，可引起从轻度呼吸道感染到瘫痪等各种症状。由于肠道病毒感染的大量存在，除非研究 IgM 片段，否则很难使用免疫学检测来区分正在进行的和以前的感染。

方法

在这项研究中，我们使用间接 ELISA 和捕获 IgM ELISA 表明，针对非结构肠道病毒蛋白 2A 和 3C 的 IgG 抗体反应可用于区分 IgM 阳性（=22）和 IgM 阴性（=20）人类患者，准确率为 83%，诊断优势比为 30。使用小鼠模型，我们确定与结构蛋白相比，蛋白酶的抗体反应持续时间较短。因此，蛋白酶抗体反应可作为急性感染的潜在标志物。