Phytomedicine, Biochemical Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Sciences, The Federal University of Technology, Akure, Nigeria, Phone: +234 803 444 5893.
Phytomedicine, Biochemical Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Sciences, The Federal University of Technology, Akure, Nigeria.
J Basic Clin Physiol Pharmacol. 2020 Jan 15;31(3):/j/jbcpp.2020.31.issue-3/jbcpp-2018-0230/jbcpp-2018-0230.xml. doi: 10.1515/jbcpp-2018-0230.
Background The physiological functions of the testis and spleen can be affected through several cellular and molecular mechanisms such as the generation of reactive oxygen species (ROS) that causes oxidative stress. This study aimed at investigating the protective effect of catechin, quercetin, and taxifolin in rotenone-induced testicular and splenetic toxicity. Methods Male Wistar rats were administered with 1.5 mg/kg rotenone (s.c.) for 10 days followed by post-treatment with catechin (5, 10, or 20 mg/kg), quercetin (5, 10, or 20 mg/kg), and taxifolin (0.25, 0.5, or 1.0 mg/kg) for 3 days (s.c.), followed by estimation of biochemical markers of oxidative stress, inflammatory activities, and tissue damage in testes and spleen. Results Exposure of rats to rotenone caused reduced body weight gain, increased organ weight, decreased glutathione level and activities of glutathione transferase and superoxide dismutase, enhanced lipid peroxidation, and increased activities of prooxidant/proinflammatory enzymes and lactate dehydrogenase, which were mitigated by post-treatment with flavonoids. In general, quercetin and taxifolin showed better activity than catechin. Conclusions Catechin, quercetin, and taxifolin ameliorated rotenone-induced weight disturbances and oxidative damage in rats, indicating their potential relevance in toxicant and pesticide-induced tissue injury.
睾丸和脾脏的生理功能可通过多种细胞和分子机制受到影响，例如产生导致氧化应激的活性氧（ROS）。本研究旨在研究儿茶素、槲皮素和taxifolin 在鱼藤酮诱导的睾丸和脾脏毒性中的保护作用。
雄性 Wistar 大鼠连续 10 天每天皮下注射 1.5mg/kg 鱼藤酮，随后连续 3 天每天皮下给予儿茶素（5、10 或 20mg/kg）、槲皮素（5、10 或 20mg/kg）和 taxifolin（0.25、0.5 或 1.0mg/kg）进行治疗，随后评估睾丸和脾脏中氧化应激、炎症活性和组织损伤的生化标志物。
暴露于鱼藤酮的大鼠体重增加减少，器官重量增加，谷胱甘肽水平和谷胱甘肽转移酶及超氧化物歧化酶活性降低，脂质过氧化增强，促氧化剂/促炎酶和乳酸脱氢酶活性增加，这些变化在给予黄酮类化合物后得到缓解。总的来说，槲皮素和 taxifolin 的活性优于儿茶素。
儿茶素、槲皮素和 taxifolin 改善了鱼藤酮诱导的大鼠体重紊乱和氧化损伤，表明它们在毒物和杀虫剂诱导的组织损伤中具有潜在的相关性。
