Barbosa Leonardo M Q, Amaral-Machado Lucas, Félix-Silva Juliana, Oliveira Wógenes N, Alencar Éverton N, Rocha Keyla B P, Medeiros Aldo da Cunha, Rocha Hugo A O, Fernandes-Pedrosa Matheus F, Egito Eryvaldo S T
Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN).
Graduation Program in Health Sciences, UFRN.
J Oleo Sci. 2020 Feb 5;69(2):133-142. doi: 10.5650/jos.ess19215. Epub 2020 Jan 16.
Bullfrog oil (BFO) is a natural product from the adipose tissue of the amphibian Rana catesbeiana Shaw, a bio-product rich in polyunsaturated fatty acids, which claims anti-inflammatory activity. The objective of this work was to evaluate the cytotoxicity and the anti-inflammatory activity of BFO using in vivo and in vitro assays. Thus, the in vitro cytotoxicity was assessed by the MTT assay. Additionally, the in vivo anti-inflammatory activity was performed by the carrageenan-induced paw edema model in Wistar rats, followed by histological analysis. Moreover, the BFO effect on inflammatory pathways was investigated by in vitro evaluation of the nitric oxide (NO) synthesis, and type-6 interleukin (IL-6) and tumor-necrosis-factor (TNF) levels. In vivo experiments showed that BFO administered by intragastric route produced a significant anti-inflammatory effect, which was as substantial as indomethacin, the positive control. Histopathological analysis confirmed these results, showing the absence of the edema and minimal signs of inflammation in the paws of rats treated with BFO. The MTT results showed that BFO at all tested concentrations had no toxic effect against a macrophage cell line, not affecting the cell viability. In addition, after 48 hours of treatment, the BFO itself and its blend with Cetiol-V (1:1) at 200 µg.mL were able to reduce the NO synthesis, and the IL-6 and TNF levels up to 35 ± 2%, 40 ± 6%, and 12 ± 3%, respectively. Therefore, these results provide unprecedented scientific evidence of the anti-inflammatory effect of BFO, suggesting its potential as a new candidate for the development of pharmaceutical products with anti-inflammatory activity.
牛蛙油（BFO）是一种源自两栖动物牛蛙（Rana catesbeiana Shaw）脂肪组织的天然产物，是一种富含多不饱和脂肪酸的生物制品，具有抗炎活性。本研究的目的是通过体内和体外试验评估牛蛙油的细胞毒性和抗炎活性。因此，采用MTT法评估体外细胞毒性。此外，通过角叉菜胶诱导的Wistar大鼠足肿胀模型进行体内抗炎活性试验，随后进行组织学分析。此外，通过体外评估一氧化氮（NO）合成、6型白细胞介素（IL-6）和肿瘤坏死因子（TNF）水平，研究了牛蛙油对炎症通路的影响。体内实验表明，经胃内途径给予牛蛙油产生了显著的抗炎作用，与阳性对照吲哚美辛的效果相当。组织病理学分析证实了这些结果，显示用牛蛙油处理的大鼠足部没有水肿且炎症迹象 minimal。MTT结果表明，所有测试浓度的牛蛙油对巨噬细胞系均无毒性作用，不影响细胞活力。此外，处理48小时后，牛蛙油本身及其与十六醇（1:1）的混合物在200 µg.mL时能够分别将NO合成、IL-6和TNF水平降低35±2%、40±6%和12±3%。因此，这些结果为牛蛙油的抗炎作用提供了前所未有的科学证据，表明其作为具有抗炎活性的药品开发新候选物的潜力。
