Graduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Chemistry Institute, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Int J Nanomedicine. 2021 Nov 2;16:7353-7367. doi: 10.2147/IJN.S318018. eCollection 2021.
Although bullfrog oil (BFO) exerts anti-inflammatory effects, it has undesirable properties limiting its use.
BFO nanocapsules (BFONc) were produced through nanoprecipitation, and their physicochemical and morphological properties were characterized. To evaluate the biocompatibility of the formulation, a mitochondrial activity evaluation assay was conducted, and cell uptake was assessed. The in vitro anti-inflammatory activity was evaluated by measuring reactive oxygen species (ROS), nitric oxide (NO), type-6 interleukin (IL-6), and tumor necrosis factor (TNF) levels. The in vivo anti-inflammatory effect was assessed by quantifying myeloperoxidase (MPO) levels using the carrageenan-induced paw edema model.
BFONc showed a particle size of 233 ± 22 nm, a polydispersity index of 0.17 ± 0.03, and a zeta potential of -34 ± 2.6mV. BFONc revealed remarkable biocompatibility and did not induce changes in cell morphology. Furthermore, BFONc decreased ROS levels by 81 ± 4%; however, NO level increased by 72 ± 18%. TNF and IL-6 levels were reduced by approximately 10% and 90%, respectively. Significant in vivo anti-inflammatory activity was observed compared to dexamethasone. MPO levels were reduced up to 2 MPOs/mg.
Taken together, the results pointed out the remarkable biocompatibility and anti-inflammatory effects of BFONc.
尽管牛蛙油（BFO）具有抗炎作用，但它也有一些不理想的性质限制了其应用。
通过纳米沉淀法制备牛蛙油纳米胶囊（BFONc），并对其理化和形态特性进行了表征。为了评估该制剂的生物相容性，进行了线粒体活性评估实验，并评估了细胞摄取情况。通过测量活性氧（ROS）、一氧化氮（NO）、白细胞介素-6（IL-6）和肿瘤坏死因子（TNF）的水平来评估体外抗炎活性。通过用角叉菜胶诱导的爪肿胀模型来定量髓过氧化物酶（MPO）水平来评估体内抗炎效果。
BFONc 的粒径为 233±22nm，多分散指数为 0.17±0.03，zeta 电位为-34±2.6mV。BFONc 表现出显著的生物相容性，不会引起细胞形态的变化。此外，BFONc 将 ROS 水平降低了 81±4%；然而，NO 水平增加了 72±18%。TNF 和 IL-6 水平分别降低了约 10%和 90%。与地塞米松相比，观察到显著的体内抗炎活性。MPO 水平降低了高达 2MPO/mg。
综上所述，结果表明 BFONc 具有显著的生物相容性和抗炎作用。
