Department of orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Cell Cycle. 2020 Feb;19(3):326-338. doi: 10.1080/15384101.2019.1710916. Epub 2020 Jan 16.
: MicroRNAs (miRNAs) have been demonstrated to engage in the nerve injury, while the effect of microRNA-192-5p (miR-192-5p) on the nerve repair has not yet been well understood. This study is performed to investigate how miR-192-5p affects nerve repair in rats with peripheral nerve injury by regulating X-linked inhibitor of apoptosis protein (XIAP).: The rat model of left sciatic nerve injury was established, and the expression of miR-192-5p was then detected. A series of experiments were conducted to investigate the role of miR-192-5p on nerve repair in rats with peripheral nerve injury. The expression of apoptosis-related proteins (Caspase-3, Bax and Bcl-2) and nerve repair factors (NGF, BDNF, and GAP-43) was measured. Bioinformatics analysis and dual-luciferase reporter gene assay confirmed the targeting relationship between miR-192-5p and XIAP.: MiR-192-5p inhibition promoted the recovery of sensory function and the recovery and regeneration in rats with sciatic nerve injury. MiR-192-5p inhibition promoted the recovery of muscle atrophy caused by nerve injury. MiR-192-5p inhibition inhibited neuronal apoptosis by affecting the expression of apoptosis-related proteins and promoted the recovery of nerve function by elevating the expression of nerve repair factors induced by peripheral nerve injury. Bioinformatics analysis and dual-luciferase reporter gene assay confirmed that XIAP was a target gene of miR-192-5p.: This study demonstrates that miR-192-5p inhibition can up-regulate the expression of XIAP, decrease the apoptosis of nerve cells, and promote the repair and regeneration of peripheral nerve injury.
: 微小 RNA（miRNA）已被证明参与神经损伤，而微小 RNA-192-5p（miR-192-5p）对神经修复的影响尚未得到很好的理解。本研究旨在通过调节 X 连锁凋亡抑制蛋白（XIAP）来研究 miR-192-5p 如何影响周围神经损伤大鼠的神经修复。: 建立了大鼠左侧坐骨神经损伤模型，然后检测 miR-192-5p 的表达。进行了一系列实验来研究 miR-192-5p 对周围神经损伤大鼠神经修复的作用。测量了凋亡相关蛋白（Caspase-3、Bax 和 Bcl-2）和神经修复因子（NGF、BDNF 和 GAP-43）的表达。生物信息学分析和双荧光素酶报告基因检测证实了 miR-192-5p 与 XIAP 之间的靶向关系。: miR-192-5p 抑制促进了感觉功能的恢复以及坐骨神经损伤大鼠的恢复和再生。miR-192-5p 抑制促进了神经损伤引起的肌肉萎缩的恢复。miR-192-5p 抑制通过影响凋亡相关蛋白的表达来抑制神经元凋亡，并通过提高周围神经损伤诱导的神经修复因子的表达来促进神经功能的恢复。生物信息学分析和双荧光素酶报告基因检测证实，XIAP 是 miR-192-5p 的靶基因。: 本研究表明，miR-192-5p 抑制可以上调 XIAP 的表达，减少神经细胞凋亡，促进周围神经损伤的修复和再生。
