Molecular and Biomedical Sciences, The University of Maine, Orono, ME, 04469, USA; Aquaculture Research Institute, The University of Maine, Orono, ME, 04469, USA.
Aquaculture Research Institute, The University of Maine, Orono, ME, 04469, USA.
Fish Shellfish Immunol. 2020 Mar;98:296-300. doi: 10.1016/j.fsi.2020.01.023. Epub 2020 Jan 14.
The ectoparasite, Lepeophtheirus salmonis (Kroyer 1837), is effective at avoiding elimination from its host, Atlantic salmon, Salmo salar L., by inhibiting the recruitment of immune cells to the site of attachment. In other ectoparasitic arthropods, numerous factors have been identified that bind or neutralize chemokines preventing their interaction with receptors on the surfaces of immune cells. To determine if L. salmonis is utilizing a similar mechanism of immune modulation, the chemotactic activity of peripheral blood leukocytes (PBL) to leukotriene B4 (LTB4) and the secreted/excreted products (SEPs) of the sea louse were investigated in vitro. The results showed that incubation of LTB4 with SEPs reduced leukocyte migration compared to LTB4 immune stimulation alone. Data suggests that one of the mechanisms L. salmonis may be using to regulate immune cell recruitment in Atlantic salmon is by inhibiting or neutralizing the activity of chemokines.
外寄生虫，鲑鱼海虱（Kroyer 1837），通过抑制免疫细胞向附着部位募集，有效地避免了从宿主大西洋鲑（Salmo salar L.）中被清除。在其他外寄生节肢动物中，已经确定了许多因素可以结合或中和趋化因子，从而阻止它们与免疫细胞表面的受体相互作用。为了确定鲑鱼海虱是否利用了类似的免疫调节机制，本研究在体外研究了外周血白细胞（PBL）对白细胞三烯 B4（LTB4）和海虱分泌/排泄产物（SEPs）的趋化活性。结果表明，与单独的 LTB4 免疫刺激相比，LTB4 与 SEPs 孵育可降低白细胞迁移。数据表明，鲑鱼海虱可能用于调节大西洋鲑免疫细胞募集的机制之一是通过抑制或中和趋化因子的活性。
