Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong, 266237, China.
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong, 266237, China.
Fish Shellfish Immunol. 2020 Mar;98:245-254. doi: 10.1016/j.fsi.2020.01.019. Epub 2020 Jan 13.
ATPase Inhibitory Factor 1 (IF1) is a mitochondrial protein that functions as a physiological inhibitor of FF-ATP synthase. In the present study, a mitochondrial ATPase inhibitor factor 1 (MjATPIF1) was identified from kuruma shrimp (Marsupenaeus japonicus), which was demonstrated to participate in the viral immune reaction of white spot syndrome virus (WSSV). MjATPIF1 contained a mitochondrial ATPase inhibitor (IATP) domain, and was widely distributed in hemocytes, heart, hepatopancreas, gills, stomach, and intestine of shrimp. MjATPIF1 transcription was upregulated in hemocytes and intestines by WSSV. WSSV replication decreased after MjATPIF1 knockdown by RNA interference and increased following recombinant MjATPIF1 protein injection. Further study found that MjATPIF1 promoted the production of superoxide and activated the transcription factor nuclear factor kappa B (NF-κB, Dorsal) to induce the transcription of WSSV RNAs. These results demonstrate that MjATPIF1 benefits WSSV replication in kuruma shrimp by inducing superoxide production and NF-κB activation.
ATP 酶抑制因子 1（IF1）是一种线粒体蛋白，作为 FF-ATP 合酶的生理抑制剂发挥作用。本研究从日本对虾（Marsupenaeus japonicus）中鉴定出线粒体 ATP 酶抑制剂因子 1（MjATPIF1），该蛋白被证明参与了白斑综合征病毒（WSSV）的病毒免疫反应。MjATPIF1 含有一个线粒体 ATP 酶抑制剂（IATP）结构域，广泛分布于虾的血细胞、心脏、肝胰腺、鳃、胃和肠道中。WSSV 感染后，MjATPIF1 在血细胞和肠组织中转录上调。通过 RNA 干扰敲低 MjATPIF1 后，WSSV 的复制减少，而注射重组 MjATPIF1 蛋白后则增加。进一步的研究发现，MjATPIF1 通过诱导超氧阴离子的产生和激活转录因子核因子 kappa B（NF-κB，Dorsal）来诱导 WSSV RNA 的转录，从而促进超氧阴离子的产生。这些结果表明，MjATPIF1 通过诱导超氧阴离子的产生和 NF-κB 的激活，有利于白斑综合征病毒在日本对虾中的复制。
