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sORF 编码的肽段、ATP 合酶亚基促进虾类 WSSV 复制。

The sORF-Encoded Peptides, ATP Synthase Subunits, Facilitate WSSV Duplication in Shrimp.

机构信息

Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, China.

Department of Biopharmacy, School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, China.

出版信息

Viruses. 2022 Nov 4;14(11):2449. doi: 10.3390/v14112449.

DOI:10.3390/v14112449
PMID:36366547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9692589/
Abstract

Short open reading frames (sORFs) are a newly identified family of genes, and the functions of most sORF genes and their encoded peptides (SEPs) are still unknown. In this study, two ATP synthase subunits were identified in kuruma shrimp () as SEPs, namely ATP5I and ATP5L. They were widely distributed in all of the tested tissues of shrimp and upregulated in hemocytes and intestines in response to WSSV challenge. The injection of recombinant proteins (rATP5I and rATP5L) increased the expression of and , while the knockdown of and decreased the expression of and . All of the results suggest that ATP5I and ATP5L were beneficial for WSSV replication. Further exploration found that and RNAi significantly improved the shrimp survival rates, reduced ATP production, and upregulated the expression of antimicrobial peptide genes post viral challenge, and the two ATPase subunits and Relish negatively regulated each other. These results reveal that ATP5I and ATP5L facilitated WSSV duplication by regulating the production of ATP contents and the expression of antimicrobial peptide genes in shrimp.

摘要

短开放阅读框 (sORFs) 是一个新发现的基因家族，大多数 sORF 基因及其编码的肽 (SEPs) 的功能仍然未知。在这项研究中，从虾夷马粪海胆中鉴定出两个 ATP 合酶亚基作为 SEPs，即 ATP5I 和 ATP5L。它们广泛分布于虾的所有检测组织中，并在 WSSV 攻击时在上皮细胞和肠道中上调。重组蛋白 (rATP5I 和 rATP5L) 的注射增加了 和 的表达，而 和 的敲低降低了 和 的表达。所有结果表明 ATP5I 和 ATP5L 有利于 WSSV 复制。进一步的探索发现， 和 的 RNAi 显著提高了虾的存活率，降低了 ATP 的产生，并在病毒攻击后上调了抗菌肽基因的表达，这两个 ATP 酶亚基和 Relish 相互负调控。这些结果表明，ATP5I 和 ATP5L 通过调节虾中 ATP 含量的产生和抗菌肽基因的表达来促进 WSSV 复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/57db4bded101/viruses-14-02449-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/2312bf575f16/viruses-14-02449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/56226c9bb15a/viruses-14-02449-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/e0dcfffac438/viruses-14-02449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/4d04a5e688c1/viruses-14-02449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/d477d2f3b3a9/viruses-14-02449-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/31a4e02c0c9a/viruses-14-02449-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/57db4bded101/viruses-14-02449-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/2312bf575f16/viruses-14-02449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/56226c9bb15a/viruses-14-02449-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/e0dcfffac438/viruses-14-02449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/4d04a5e688c1/viruses-14-02449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/d477d2f3b3a9/viruses-14-02449-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/31a4e02c0c9a/viruses-14-02449-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab77/9692589/57db4bded101/viruses-14-02449-g007.jpg

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