Wang Dandan, Xu Duoduo, Zhang Yanqiu, Zhao Daqing, Wang Mingxing
Research Center of Traditional Chinese medicine, Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, China.
Jilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China.
J Food Biochem. 2020 Mar;44(3):e13135. doi: 10.1111/jfbc.13135. Epub 2020 Jan 20.
A novel oligosaccharide showed that protection against LPS-induced Caco-2 cells was purified from the mycelium of Hericium erinaceus (HE). WEP-1 is mainly composed of neutral monosaccharides with molecular weight of 4,010 Da and of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1. The structure of WEP-1 includes α-D-Glc (1 → 3) and β-D-Gal (1 → 3) as the backbone with β-D-Glc (1 → 3) as branches attached to the C-4 position and β-D-Man as a terminal residue. The oligosaccharide reduced acetic acid-induced colonic mucosa injury in rats. It also showed significant protection against LPS-induced Caco-2 cells via the TLR4/NF-κB pathway. PRACTICAL APPLICATIONS: In the study, the oligosaccharide from HE has the potential to be developed into functional foods or medicines for the treatment of intestinal diseases. The protection against LPS-induced Caco-2 cells via the TLR4/NF-κB pathway may be a key target for the pharmacological activity of HE.
一种新型寡糖显示出对脂多糖诱导的Caco-2细胞具有保护作用，它是从猴头菇（HE）的菌丝体中纯化得到的。WEP-1主要由中性单糖组成，分子量为4010 Da，其甘露糖、葡萄糖和半乳糖的摩尔比为1.2:16.9:1。WEP-1的结构包括以α-D-葡萄糖（1→3）和β-D-半乳糖（1→3）为主链，β-D-葡萄糖（1→3）作为分支连接在C-4位，β-D-甘露糖作为末端残基。该寡糖减轻了大鼠乙酸诱导的结肠黏膜损伤。它还通过TLR4/NF-κB途径对脂多糖诱导的Caco-2细胞显示出显著的保护作用。实际应用：在该研究中，来自猴头菇的寡糖有潜力被开发成治疗肠道疾病的功能性食品或药物。通过TLR4/NF-κB途径对脂多糖诱导的Caco-2细胞的保护作用可能是猴头菇药理活性的关键靶点。
