Wang Mingxing, Gao Yang, Xu Duoduo, Gao Qipin
Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130117, China; International Collaborative Research Center, Changchun University of Chinese Medicine, Changchun 130117, China.
Research and Development Center, Changchun University of Chinese Medicine, Changchun 130117, China.
Int J Biol Macromol. 2015 Nov;81:656-61. doi: 10.1016/j.ijbiomac.2015.08.043. Epub 2015 Aug 24.
A polysaccharide named EP-1 was found by screening cultured mycelium of Hericium erinaceus, which was extracted and subjected to precipitation with ethanol, hollow-fiber ultrafiltration and ion-exchange chromatography. The polysaccharide has a molecular weight of approximately 3100Da and is composed of glucose, mannose and galactose, thus being a heteroglycan. EP-1 has a backbone of α-d-Glc(1→3) and β-d-Glc(1→3). The β-d-Glc(1→3) and α-d-Gal-(1→3) were regarded as branches attached to the C-4 position. The α-d-Man was regarded as a terminal residue. The anti-CAG activity was evaluated in experimental systems using a cell model for identification. The polysaccharide significantly inhibited the growth of MC cells obtained from human gastric mucosa epithelium (GES-1) cells transformed by MNNG, which were used as a chronic atrophic gastritis cell model. It also interfered with the MC cells by inducing cell cycle arrest. Thus, EP-1 shows potential for the development of new functional foods and drugs.
通过筛选猴头菇培养菌丝体发现了一种名为EP-1的多糖，该多糖经过乙醇沉淀、中空纤维超滤和离子交换色谱法进行提取和处理。该多糖的分子量约为3100Da，由葡萄糖、甘露糖和半乳糖组成，因此是一种杂聚糖。EP-1具有α-d-Glc(1→3)和β-d-Glc(1→3)的主链。β-d-Glc(1→3)和α-d-Gal-(1→3)被视为连接在C-4位的分支。α-d-Man被视为末端残基。使用细胞模型在实验系统中评估抗CAG活性。该多糖显著抑制了由MNNG转化的人胃黏膜上皮（GES-1）细胞获得的MC细胞的生长，这些细胞被用作慢性萎缩性胃炎细胞模型。它还通过诱导细胞周期停滞来干扰MC细胞。因此，EP-1显示出开发新型功能性食品和药物的潜力。
