College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China.
Biosci Rep. 2020 Feb 28;40(2). doi: 10.1042/BSR20191248.
Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits.
Wnt 信号通路在毛囊（HFs）的生长和发育中起着重要作用。在这些信号分子中，Wnt10b 被证明可促进体外培养的小鼠原代皮肤上皮细胞向 HF 细胞的毛干和内根鞘分化。从雷克斯兔中分离出须状 HF 并进行体外培养，以测量毛干生长。同时，分离并体外培养真皮乳头细胞（DPCs）。通过 CCK-8 测定法评估 AdWnt10b 或 Wnt/β-Catenin 通路抑制剂 XAV939 对 DPCs 增殖的影响。并通过流式细胞术分析细胞周期。我们发现 Wnt10b 可以促进毛干伸长，而 XAV-939 处理可以消除这种现象。AdWnt10b 处理促进了 DPCs 的增殖，并诱导了 G1/S 期转变。AdWnt10b 刺激使 DPCs 中的 β-Catenin 蛋白上调。XAV-939 抑制 Wnt/β-Catenin 信号通路可降低 DPCs 的基础增殖和 Wnt10b 增强的增殖。还可以抑制 DPCs 的细胞周期进程。总之，我们的研究表明，Wnt10b 可以通过 Rex 兔中的 Wnt/β-Catenin 信号通路促进 HFs 的生长和 DPCs 的增殖。
