Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, E-08197 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, E-08197 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain; and Corresponding authors. Email:
Reprod Fertil Dev. 2020 Mar;32(6):619-628. doi: 10.1071/RD19192.
The aim of this study was to evaluate the response of donkey spermatozoa to oxidative stress induced by hydrogen peroxide, and to determine whether the presence of seminal plasma modulates the sperm response to that stress. Nine ejaculates were collected, extended in skim milk extender and split into two aliquots. Seminal plasma was removed from the first but not second aliquot. Samples were subsequently split into four aliquots supplemented with different concentrations of commercial hydrogen peroxide (0, 100 and 250µM and 50mM). Aliquots were incubated at 37°C under aerobic conditions and several sperm parameters, namely motility, viability, intracellular levels of peroxides and superoxides and mitochondrial membrane potential, were evaluated at 0, 1 and 3h. Exposure to hydrogen peroxide markedly decreased sperm motility but had much less of an effect on sperm viability, mitochondrial membrane potential and intracellular reactive oxygen species levels. A protective effect of seminal plasma against the loss of sperm motility was not apparent, but some kinetic parameters and relative levels of superoxides were better maintained when seminal plasma was present together with high concentration of hydrogen peroxide. In conclusion, oxidative stress induced by hydrogen peroxide reduces donkey sperm motility and has a less apparent effect on other sperm parameters. Finally, seminal plasma is only able to partially ameliorate the detrimental effect of this induced stress.
本研究旨在评估过氧化氢诱导的驴精子对氧化应激的反应，并确定精浆是否调节精子对这种应激的反应。收集了 9 个精液样本，用脱脂乳 extender 进行扩展，并分成两份。第一份去除了精浆，但第二份没有。随后将样本分成四等份，分别添加不同浓度的商业过氧化氢（0、100 和 250µM 和 50mM）。等分试样在 37°C 下有氧条件下孵育，并在 0、1 和 3 小时评估运动性、活力、细胞内过氧化物和超氧化物水平以及线粒体膜电位等几个精子参数。过氧化氢暴露显著降低精子运动性，但对精子活力、线粒体膜电位和细胞内活性氧水平的影响较小。精浆对精子运动性丧失的保护作用不明显，但当精浆与高浓度过氧化氢一起存在时，一些动力学参数和超氧化物的相对水平得到更好的维持。总之，过氧化氢诱导的氧化应激降低了驴精子的运动性，对其他精子参数的影响较小。最后，精浆只能部分改善这种诱导应激的有害影响。
