Agarwal A, Sharma R, Henkel R, Roychoudhury S, Sikka S C, du Plessis S, Sarda Y B, Speyer C, Nouh M, Douglas C, Kayali Z, Elshaer A, Sabanegh E
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
Department of Medical Bioscience, University of the Western Cape, Bellville, South Africa.
Andrologia. 2018 Feb;50(1). doi: 10.1111/and.12796. Epub 2017 Mar 15.
Oxidation-reduction potential (ORP) is a newer integrated measure of the balance between total oxidants (reactive oxygen species-ROS) and reductants (antioxidants) that reflects oxidative stress in a biological system. This study measures ORP and evaluates the effect of exogenous induction of oxidative stress by cumene hydroperoxide (CH) on ORP in fresh and frozen semen using the MiOXSYS Analyzer. Semen samples from healthy donors (n = 20) were collected and evaluated for sperm parameters. All samples were then flash-frozen at -80°C. Oxidative stress was induced by CH (5 and 50 μmoles/ml). Static ORP (sORP-(mV/10 sperm/ml) and capacity ORP (cORP-μC/10 sperm/ml) were measured in all samples before and after freezing. All values are reported as mean ± SEM. Both 5 and 50 μmoles/ml of CH resulted in a significant decline in per cent motility compared to control in pre-freeze semen samples. The increase in both pre-freeze and post-thaw semen samples for sORP was higher in the controls than with 50 μmoles/ml of CH. The change from pre-freeze to post-thaw cORP was comparable. The system is a simple, sensitive and portable tool to measure the seminal ORP and its dynamic impact on sperm parameters in both fresh and frozen semen specimens.
氧化还原电位（ORP）是一种较新的综合指标，用于衡量总氧化剂（活性氧物种-ROS）和还原剂（抗氧化剂）之间的平衡，反映生物系统中的氧化应激。本研究使用MiOXSYS分析仪测量ORP，并评估异丙苯过氧化氢（CH）外源性诱导氧化应激对新鲜和冷冻精液中ORP的影响。收集来自健康供体的精液样本（n = 20）并评估精子参数。然后将所有样本在-80°C下速冻。通过CH（5和50微摩尔/毫升）诱导氧化应激。在冷冻前后测量所有样本的静态ORP（sORP-毫伏/10个精子/毫升）和容量ORP（cORP-微库仑/10个精子/毫升）。所有值均报告为平均值±标准误。与冷冻前精液样本中的对照相比，5和50微摩尔/毫升的CH均导致活力百分比显著下降。冷冻前和解冻后精液样本中，对照组sORP的增加高于50微摩尔/毫升CH处理组。冷冻前到解冻后cORP的变化相当。该系统是一种简单、灵敏且便携的工具，可用于测量精液ORP及其对新鲜和冷冻精液样本中精子参数的动态影响。
