Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Straße 6, 24118, Kiel, Germany.
Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, 76131, Karlsruhe, Germany.
Mol Nutr Food Res. 2020 Mar;64(6):e1901116. doi: 10.1002/mnfr.201901116. Epub 2020 Feb 11.
Data on resveratrol-(trans-3,5,4'-trihydroxystilbene)-induced caloric-restriction-(CR)-mimicking effects in mice receiving a high-fat diet (HFD) are contradictory. It is hypothesized that this can possibly stem from different bioactivities of resveratrol (RSV) microbial metabolites.
C57BL/6Rj mice are fed an ad-libitum HFD supplemented with RSV or its metabolites, dihydroresveratrol (DHR) and lunularin (LUN) (≈28 mg (dihydro)stilbene kg mouse per day). A 40% CR group was included in the study. While CR mice show robust changes in bodyweight and composition, hormone levels and mRNA expression, slight changes are found (more muscle, less adipose tissue) in body composition, leptin, and insulin levels in RSV-supplemented mice compared to ad libitum controls. LUN hardly and DHR does not change the hormone levels measured. Metabolome analysis of serum shows changes in CR mice but only slight, if any, changes in RSV-, DHR-, or LUN-supplemented mice compared to the controls. Evaluating the capability of RSV and its metabolites to inhibit carbohydrate-hydrolyzing enzymes in vitro, it is found that RSV reduced α-glucosidase activity to a stronger extent than DHR and LUN.
Decelerated carbohydrate breakdown by RSV may have contributed to the moderate impact of dietary RSV on mouse insulin sensitivity (lowered fasting and post-glucose-bolus insulin levels).
关于白藜芦醇（反式-3,5,4'-三羟基二苯乙烯）在高脂肪饮食（HFD）喂养的小鼠中诱导热量限制（CR）模拟作用的数据存在矛盾。据推测，这可能源于白藜芦醇（RSV）微生物代谢物的不同生物活性。
C57BL/6Rj 小鼠喂食自由摄取的 HFD，并补充 RSV 或其代谢物二氢白藜芦醇（DHR）和月桂黄素（LUN）（≈28 mg（二氢）芪/kg 小鼠/天）。研究中包括一个 40% CR 组。虽然 CR 小鼠的体重和组成、激素水平和 mRNA 表达发生了明显变化，但与自由摄取的对照相比，补充 RSV 的小鼠的身体成分、瘦素和胰岛素水平仅发生轻微变化（更多肌肉，更少脂肪组织）。LUN 几乎不改变所测量的激素水平，DHR 则没有改变。与对照相比，血清代谢组学分析显示 CR 小鼠发生了变化，但补充 RSV、DHR 或 LUN 的小鼠仅发生轻微变化。评估 RSV 及其代谢物在体外抑制碳水化合物水解酶的能力时，发现 RSV 降低α-葡萄糖苷酶活性的程度强于 DHR 和 LUN。
RSV 减缓碳水化合物的分解可能导致饮食 RSV 对小鼠胰岛素敏感性的影响适中（降低空腹和葡萄糖后胰岛素水平）。
