Menaya J, Parrilla R, Ayuso M S
Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.
Biochem J. 1988 Sep 15;254(3):773-9. doi: 10.1042/bj2540773.
Vasopressin was found to be an effective inhibitor of protein labelling in isolated liver cells. Its effect shows the following distinct characteristics: (1) in contrast with alpha-adrenergic agonists, its effect is observable under a wide range of cellular Ca2+-loading conditions; (2) it is not influenced by the nutritional state of the animal. The lack of vasopressin effect on valine production, and its ability to decrease protein labelling from near-saturation concentrations of [3H]valine, indicate that the observed variations in protein labelling reflect actual changes in the rate of protein synthesis. The action of vasopressin is primarily exerted on the initiation step of protein synthesis and this effect is accompanied by a decreased activity of eukaryotic initiation factor 2. Activators of protein kinase C showed similar but not additive effects on protein synthesis, as did vasopressin. It seems plausible to conclude that protein kinase C activation may play an important regulatory role in hepatic protein synthesis as a transducer of hormonal and perhaps other type of signals.
血管加压素被发现是分离的肝细胞中蛋白质标记的有效抑制剂。其作用表现出以下明显特征：（1）与α-肾上腺素能激动剂不同，在广泛的细胞钙负载条件下均可观察到其作用；（2）不受动物营养状态的影响。血管加压素对缬氨酸产生没有影响，并且它能够从接近饱和浓度的[3H]缬氨酸降低蛋白质标记，这表明观察到的蛋白质标记变化反映了蛋白质合成速率的实际变化。血管加压素的作用主要施加于蛋白质合成的起始步骤，并且这种作用伴随着真核起始因子2活性的降低。蛋白激酶C激活剂对蛋白质合成表现出与血管加压素相似但非相加的作用。可以合理地得出结论，蛋白激酶C激活作为激素及可能其他类型信号的转导者，可能在肝脏蛋白质合成中起重要的调节作用。
