利用rRNA-纤维素色谱法从大鼠肝脏微粒体中快速分离纯化蛋白质合成起始因子eIF-2
The use of rRNA-cellulose chromatography in the rapid isolation of homogeneous protein synthesis initiation factor eIF-2 from rat liver microsomes.
作者信息
Nygård O, Westermann P, Hultin T
出版信息
Biochim Biophys Acta. 1980 Jun 27;608(1):196-200. doi: 10.1016/0005-2787(80)90148-3.
PMID:7388032
Abstract
A rapid and efficient procedure is described for preparing homogeneous polypeptide chain initiation factor eIF-2 from rat liver microsomal salt wash. The method involves ammonium sulfate fractionation, affinity chromatography on rRNA-cellulose and DEAE-cellulose chromatography. The preparation is highly active in initiation complex formation.
摘要
本文描述了一种从大鼠肝脏微粒体盐洗物中制备均一的多肽链起始因子eIF-2的快速高效方法。该方法包括硫酸铵分级分离、rRNA-纤维素亲和层析和DEAE-纤维素层析。所制备的产物在起始复合物形成方面具有高活性。
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