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利用rRNA-纤维素色谱法从大鼠肝脏微粒体中快速分离纯化蛋白质合成起始因子eIF-2

The use of rRNA-cellulose chromatography in the rapid isolation of homogeneous protein synthesis initiation factor eIF-2 from rat liver microsomes.

作者信息

Nygård O, Westermann P, Hultin T

出版信息

Biochim Biophys Acta. 1980 Jun 27;608(1):196-200. doi: 10.1016/0005-2787(80)90148-3.

DOI:10.1016/0005-2787(80)90148-3
PMID:7388032
Abstract

A rapid and efficient procedure is described for preparing homogeneous polypeptide chain initiation factor eIF-2 from rat liver microsomal salt wash. The method involves ammonium sulfate fractionation, affinity chromatography on rRNA-cellulose and DEAE-cellulose chromatography. The preparation is highly active in initiation complex formation.

摘要

本文描述了一种从大鼠肝脏微粒体盐洗物中制备均一的多肽链起始因子eIF-2的快速高效方法。该方法包括硫酸铵分级分离、rRNA-纤维素亲和层析和DEAE-纤维素层析。所制备的产物在起始复合物形成方面具有高活性。

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The use of rRNA-cellulose chromatography in the rapid isolation of homogeneous protein synthesis initiation factor eIF-2 from rat liver microsomes.利用rRNA-纤维素色谱法从大鼠肝脏微粒体中快速分离纯化蛋白质合成起始因子eIF-2
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引用本文的文献

1
Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3 small ribosomal subunit, as shown by cross-linking experiments.交联实验表明,真核起始因子eIF-3的一个亚基与二元复合物eIF-3小核糖体亚基中的18S核糖体RNA发生特异性相互作用。
Nucleic Acids Res. 1982 Feb 25;10(4):1327-34. doi: 10.1093/nar/10.4.1327.
2
Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit.在真核生物抑制复合物eIF-2.GMPPCP.Met-tRNAf.小核糖体亚基中,甲硫氨酰-tRNAf与eIF-2β以及核糖体蛋白S3a和S6发生交联。
Nucleic Acids Res. 1981 May 25;9(10):2387-96. doi: 10.1093/nar/9.10.2387.
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Interaction of a cellular 57-kilodalton protein with the internal translation initiation site of foot-and-mouth disease virus.一种细胞57千道尔顿蛋白与口蹄疫病毒内部翻译起始位点的相互作用。
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