Tahernia Mehdi, Mohammadifar Maedeh, Choi Seokheun
Bioelectronics & Microsystems Laboratory, Department of Electrical & Computer Engineering, State University of New York-Binghamton, Binghamton, NY 13902, USA.
Micromachines (Basel). 2020 Jan 17;11(1):99. doi: 10.3390/mi11010099.
We developed an innovative paper-based platform for high-throughput culturing, trapping, and monitoring of . A 96-well array was readily fabricated by placing a nutrient-replenished paper substrate on a micromachined 96-well plastic frame, providing high-throughput 3D culturing environments and in situ analysis of the worms. The paper allows to pass through the porous and aquatic paper matrix until the worms grow and reach the next developmental stages with the increased body size comparable to the paper pores. When the diameter of becomes larger than the pore size of the paper substrate, the worms are trapped and immobilized for further high-throughput imaging and analysis. This work will offer a simple yet powerful technique for high-throughput sorting and monitoring of at a different larval stage by controlling and choosing different pore sizes of paper. Furthermore, we developed another type of 3D culturing system by using paper-like transparent polycarbonate substrates for higher resolution imaging. The device used the multi-laminate structure of the polycarbonate layers as a scaffold to mimic the worm's 3D natural habitats. Since the substrate is thin, mechanically strong, and largely porous, the layered structure allowed to move and behave freely in 3D and promoted the efficient growth of both and their primary food, . The transparency of the structure facilitated visualization of the worms under a microscope. Development, fertility, and dynamic behavior of in the 3D culture platform outperformed those of the standard 2D cultivation technique.
我们开发了一种创新的纸质平台，用于高通量培养、捕获和监测……通过将营养补充的纸质基质放置在微加工的96孔塑料框架上，可轻松制作出96孔阵列，从而提供高通量的3D培养环境并对蠕虫进行原位分析。纸张允许……穿过多孔的水生纸质基质，直到蠕虫生长并达到下一个发育阶段，其体型增大到与纸张孔隙相当。当……的直径大于纸质基质的孔径时，蠕虫就会被捕获并固定，以便进行进一步的高通量成像和分析。这项工作将提供一种简单而强大的技术，通过控制和选择不同孔径的纸张，对处于不同幼虫阶段的……进行高通量分类和监测。此外，我们通过使用类似纸张的透明聚碳酸酯基板开发了另一种3D培养系统，用于更高分辨率的成像。该装置利用聚碳酸酯层的多层层压结构作为支架，以模拟蠕虫的3D自然栖息地。由于基板薄、机械强度高且多孔，分层结构允许……在3D中自由移动和活动，并促进了……及其主要食物……的高效生长。该结构的透明度便于在显微镜下观察蠕虫。在3D培养平台中，……的发育、繁殖力和动态行为优于标准的2D培养技术。
