Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), via Archirafi 32, 90123 Palermo, Italy.
IZS Istituto Zooprofilattico Sperimentale della Sicilia via Gino Marinuzzi, 3, 90129 Palermo, Italy.
Molecules. 2020 Jan 17;25(2):383. doi: 10.3390/molecules25020383.
The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five isolates from a variety of foods and five isolates from a farm. The isolates were positive for extended spectrum β-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 μg/mL. An interesting activity of interference with biofilm formation of and was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 μg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old biofilms at concentrations of 500 or 800 µg/mL, corresponding to 2× MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack biofilm formation was also observed at a concentration of 100 µg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting.
本研究的主要目的是评估百里酚控制食品加工设施环境污染的功效。作为一种防止浮游生物和细菌生物膜生长的试剂，对百里酚的效果进行了测试。这 25 株分离株来自多种食品，5 株分离株来自一个农场，均为产Extended spectrum β-lactamase (ESBL)基因的阳性。所有分离株和参考菌株对最低抑菌浓度（MIC）值在 250 至 800μg/ml 之间的百里酚均敏感。在亚 MIC 浓度（200、100、75 和 50μg/ml）下，百里酚对 和 生物膜形成具有有趣的干扰活性。在浓度为 500 或 800μg/ml（相当于 2×MIC）时，针对六种分离株中的游离形式，测定了对 24 小时形成的生物膜的抗生物膜活性，范围为 59.71%至 66.90%，这六种分离株被选为测试菌株中生物膜形成能力最好或中等的菌株。在使用不锈钢模型模拟食品工业表面的情况下，在浓度为 100μg/ml（相当于 1/4 MIC）时，也观察到了百里酚对 生物膜形成的攻击作用。本研究提供了在食品加工环境中使用百里酚的信息。
