Gama-Lara Sergio Arturo, Pérez Mendoza Martha Stephanie, Vilchis-Nestor Alfredo Rafael, Natividad Reyna
Engineering department, campus Toluca, Universidad del Valle de México, De Las Palmas 439, San Jorge Pueblo Nuevo, Metepec 52164, Mexico.
Centro Conjunto de Investigación en Química Sustentable UAEM-UNAM, Universidad Autónoma del Estado de México, Carretera Toluca-Atlacomulco Km 14.5, San Cayetano, Toluca 50200, Mexico.
Materials (Basel). 2020 Jan 18;13(2):462. doi: 10.3390/ma13020462.
Bionanotechnology is a relatively new term that implies the use of some biological material or organisms in order to prepare nanosystems or nanoparticles. This work presents the preparation and bactericide application of a sustainable nanometric system (silver nanoparticles) using a waste biological support (bovine bone powder). This system was prepared by the method of metallic salt reduction, using NaBH as reducing agent and AgNO as metallic salt. Two silver contents were analyzed, 1% and 5% weight. The latter was found to be more efficient than the former. Transmission electronic microscopy shows an average size of 10.5 ± 3.3 nm and quasi-sphere morphology. The antimicrobial assay shows that a 5% weight content of silver had a bactericide effect for at 46.8 min of exposure. The minimum inhibitory concentration (MIC) of silver nanoparticles supported on bovine bone powder for was 7.5 µg/mL. The biocomposite exhibits a specific antibacterial kinetics constant (k) of 0.1128 min and decimal reduction time (DRT) of 20.39 min for . Thus, it was concluded that a biocomposite was prepared with a biodegradable, waste, and low-cost support, under mild conditions (room temperature and atmospheric pressure) and using water as solvent.
生物纳米技术是一个相对较新的术语，它意味着使用某些生物材料或生物体来制备纳米系统或纳米颗粒。这项工作展示了一种可持续纳米系统（银纳米颗粒）的制备及其杀菌应用，该系统使用了一种废弃生物载体（牛骨粉）。该系统通过金属盐还原法制备，使用硼氢化钠（NaBH）作为还原剂，硝酸银（AgNO）作为金属盐。分析了两种银含量，分别为1%和5%（重量）。结果发现，5%（重量）的银含量比1%（重量）的更有效。透射电子显微镜显示其平均尺寸为10.5±3.3纳米，形态为准球形。抗菌试验表明，5%（重量）的银含量在暴露46.8分钟时具有杀菌效果。负载在牛骨粉上的银纳米颗粒对[具体菌种]的最低抑菌浓度（MIC）为7.5微克/毫升。该生物复合材料对[具体菌种]表现出特定的抗菌动力学常数（k）为0.1128分钟⁻¹，杀菌时间（DRT）为20.39分钟。因此，可以得出结论，该生物复合材料是在温和条件（室温及大气压）下，以水为溶剂，使用可生物降解、废弃且低成本的载体制备而成。
