Team 'Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism' EA 7270, Université de Bourgogne Franche-Comté/Inserm, 21000 Dijon, France.
Int J Mol Sci. 2020 Jan 18;21(2):641. doi: 10.3390/ijms21020641.
In the case of neurodegenerative pathologies, the therapeutic arsenal available is often directed towards the consequences of the disease. The purpose of this study is, therefore, to evaluate the ability of docosahexaenoic acid (DHA), a molecule present in certain foods and considered to have health benefits, to inhibit the cytotoxic effects of very long-chain fatty acids (C24:0, C26:0), which can contribute to the development of some neurodegenerative diseases. The effect of DHA (50 µM) on very long-chain fatty acid-induced toxicity was studied by several complementary methods: phase contrast microscopy to evaluate cell viability and morphology, the MTT test to monitor the impact on mitochondrial function, propidium iodide staining to study plasma membrane integrity, and DHE staining to measure oxidative stress. A Western blot assay was used to assess autophagy through modification of LC3 protein. The various experiments were carried out on the cellular model of 158N murine oligodendrocytes. In 158N cells, our data establish that DHA is able to inhibit all tested cytotoxic effects induced by very long-chain fatty acids.
在神经退行性病变的情况下，可用的治疗手段通常针对疾病的后果。因此，本研究旨在评估二十二碳六烯酸（DHA）的能力，DHA 是一种存在于某些食物中的分子，被认为具有健康益处，可抑制可能导致某些神经退行性疾病发展的超长链脂肪酸（C24:0、C26:0）的细胞毒性作用。通过几种互补的方法研究了 DHA（50µM）对超长链脂肪酸诱导的毒性的影响：相差显微镜评估细胞活力和形态，MTT 试验监测对线粒体功能的影响，碘化丙啶染色研究质膜完整性，DHE 染色测量氧化应激。通过 LC3 蛋白的修饰，使用 Western blot 分析评估自噬。各种实验均在 158N 鼠少突胶质细胞的细胞模型上进行。在 158N 细胞中，我们的数据表明 DHA 能够抑制由超长链脂肪酸引起的所有测试的细胞毒性作用。
