在越生芽孢杆菌 151-6 和海生盐单胞菌 151-25 中，由 P 型 ATP 酶基因和负责镉抗性的转录调节基因组成的操纵子。
An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25.
机构信息
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Life Science and Technology, Jinan University, Guangzhou, Guangdong, China.
出版信息
BMC Microbiol. 2020 Jan 21;20(1):18. doi: 10.1186/s12866-020-1705-2.
BACKGROUND
Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd resistance.
RESULTS
In this study, we isolated two different Cd resistance Bacillus sp. strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25, which could be grown in the presence of Cd at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome analysis under cadmium stress, and other related experiments, a gene cluster in plasmid p25 was found to be a major contributor to Cd resistance in B. marisflavi 151-25. The cluster in p25 contained orf4802 and orf4803 which encodes an ATPase transporter and a transcriptional regulator protein, respectively. Although 151-6 has much lower Cd resistance than 151-25, they contained similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111, orf4112 and orf4113, which encodes an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to play a major role on the Cd resistance for B. vietamensis 151-6.
CONCLUSIONS
This work described cadmium resistance mechanisms in newly isolated Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus and analysis of transcriptome under Cd induction, we inferred that the mechanisms of cadmium resistance in B. marisflavi 151-25 was as same as the cad system in S. aureus. Although Bacillus vietamensis 151-6 also had the similar gene cluster to B. marisflavi 151-25 and S. aureus, its transcriptional regulatory mechanism of cadmium resistance was not same. This study explored the cadmium resistance mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 and has expanded our understanding of the biological effects of cadmium.
背景
镉（Cd）是大多数微生物的剧毒重金属。许多细菌已经产生了镉抗性。
结果
在这项研究中，我们分离到两种不同的镉抗性芽孢杆菌菌株，芽孢杆菌 vietamensis 151-6 和芽孢杆菌 marisflavi 151-25，它们可以在 0.3 mM 和 0.8 mM 的 Cd 浓度下生长。根据基因组测序、镉胁迫下的转录组分析和其他相关实验，发现质粒 p25 中的一个基因簇是芽孢杆菌 marisflavi 151-25 镉抗性的主要贡献者。p25 中的簇包含 orf4802 和 orf4803，分别编码 ATP 酶转运蛋白和转录调节蛋白。尽管 151-6 的 Cd 抗性比 151-25 低得多，但它们含有相似的基因簇，但位置不同。在染色体上发现了一个基因簇，包含 orf4111、orf4112 和 orf4113，它们分别编码 ATP 酶转运蛋白、镉外排系统辅助蛋白和镉抗性蛋白，这对于芽孢杆菌 vietamensis 151-6 的 Cd 抗性起着主要作用。
结论
本研究描述了新分离的芽孢杆菌 vietamensis 151-6 和芽孢杆菌 marisflavi 151-25 的镉抗性机制。基于与金黄色葡萄球菌 cad 系统（CadA-CadC）的同源性和 Cd 诱导下的转录组分析，我们推断芽孢杆菌 marisflavi 151-25 的镉抗性机制与金黄色葡萄球菌的 cad 系统相同。尽管芽孢杆菌 vietamensis 151-6 也具有与芽孢杆菌 marisflavi 151-25 和金黄色葡萄球菌相似的基因簇，但它的镉抗性转录调节机制并不相同。本研究探索了芽孢杆菌 vietamensis 151-6 和芽孢杆菌 marisflavi 151-25 的镉抗性机制，扩展了我们对镉生物学效应的认识。
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