Turkler Can, Onat Taylan, Yildirim Engin, Kaplan Selcuk, Yazici Gulce Naz, Mammadov Renad, Sunar Mukadder
Department of Gynecology and Obstetrics, Faculty of Medicine, Erzincan Binali Yıldırım University, Turkey.
Department of Gynecology and Obstetrics, Faculty of Medicine, Bozok University, Yozgat, Turkey.
Adv Clin Exp Med. 2020 Jan;29(1):5-11. doi: 10.17219/acem/111809.
Methotrexate (MTX) is an antineoplastic agent, which increases the level of reactive oxygen species (ROS) and decreases the level of antioxidants. Lycopene, is a potent antioxidant, which is used because of its protective effect against tissue damage.
The aim of this study was to determine the effect of lycopene on ovarian MTX-induced injury in rats.
Rats (n = 36) were randomly divided into 3 equal groups: a group with MTX only (MG, n = 12), a group with lycopene and MTX (LMG, n = 12), and a healthy control group (HCG, n = 12). Then, malondialdehyde (MDA), myeloperoxidase (MPO) and total glutathione (tGSH) levels and histopathological findings were examined in the ovaries of rats. Apart from the histopathological and biochemical evaluation, the reproductive performance of the experimental groups was also examined.
Our study demonstrated that, in ovarian tissues of rats administered MTX, there was a decrease in the levels of tGSH, while MDA and MPO were increased, but it is observed that these ratios are reversed in the LMG (p < 0.05). It also has been proven that a single, high-dose use of MTX causes infertility in female rats, prolongs the gestation period and reduces the number of offspring.
Lycopene pretreatment ameliorates the MTX induced ovarian injury and infertility in rats through its antioxidative activities.
甲氨蝶呤（MTX）是一种抗肿瘤药物，它会增加活性氧（ROS）水平并降低抗氧化剂水平。番茄红素是一种强效抗氧化剂，因其对组织损伤的保护作用而被使用。
本研究旨在确定番茄红素对大鼠卵巢MTX诱导损伤的影响。
将36只大鼠随机分为3组，每组12只：仅给予MTX的组（MG组）、给予番茄红素和MTX的组（LMG组）以及健康对照组（HCG组）。然后，检测大鼠卵巢中的丙二醛（MDA）、髓过氧化物酶（MPO）和总谷胱甘肽（tGSH）水平以及组织病理学结果。除了组织病理学和生化评估外，还检测了实验组的生殖性能。
我们的研究表明，在给予MTX的大鼠卵巢组织中，tGSH水平降低，而MDA和MPO水平升高，但在LMG组中这些比例出现了逆转（p < 0.05）。还证实单次高剂量使用MTX会导致雌性大鼠不孕，延长妊娠期并减少后代数量。
番茄红素预处理通过其抗氧化活性改善了MTX诱导的大鼠卵巢损伤和不孕。
