Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan.
Research Institute of Odontology (M-RIO), Meikai University, Sakado, Japan.
Nat Prod Res. 2021 Nov;35(22):4388-4393. doi: 10.1080/14786419.2020.1716352. Epub 2020 Jan 22.
Phytochemical analysis of seeds resulted in the isolation of one new () and 23 known (－) cardenolide glycosides. The structure of was determined based on one- and two-dimensional NMR spectroscopic analysis and acid hydrolytic cleavage reaction. The effect of the cytotoxic activity of － on three human oral carcinoma cell lines was assessed. The cell lines included Ca9-22 human gingival carcinoma cells, HSC-2 human mouth carcinoma cells, HSC-4 human tongue carcinoma cells, and HGF human gingival fibroblast cells. The isolated compounds had a cytotoxic effect on the carcinoma cells with IC values ranging from 0.004 μM to 64.9 μM. The structure-activity relationship is also discussed.
种子的植物化学分析导致分离出一种新的（）和 23 种已知的（－）卡烯醇糖苷。根据一维和二维 NMR 光谱分析以及酸水解裂解反应确定了的结构。评估了－对三种人口腔癌细胞系的细胞毒性活性的影响。细胞系包括 Ca9-22 人牙龈癌细胞、HSC-2 人口腔癌细胞、HSC-4 人舌癌细胞和 HGF 人牙龈成纤维细胞。分离的化合物对癌细胞具有细胞毒性作用，IC 值范围为 0.004 μM 至 64.9 μM。还讨论了结构-活性关系。
