Williams R, Rush R A
Department of Physiology, School of Medicine, Flinders University of South Australia, Bedford Park.
Brain Res. 1988 Oct 25;463(1):21-7. doi: 10.1016/0006-8993(88)90522-7.
The immunocytochemical localization of nerve growth factor (NGF) in the embryonic mouse has been examined using correlative light and electron microscope procedures. In flat-embedded thick sections, primary sensory neurons of the olfactory pathway were clearly visible by their high level of NGF-like immunoreactivity. When ultrathin sections were examined under the electron microscope, the HRP reaction product present in the nasal epithelium was confined to the cytoplasm of the chemoreceptor cells, including the dendrites projecting into the nasal cavity. The axons projecting to the olfactory bulb and axon bundles within the bulb were also stained specifically for NGF. The reaction product did not appear to be associated with any particular subcellular structure. Neither the columnar supporting cells nor the basal precursor cells showed any evidence of immunoreactivity. No evidence was found for the presence of NGF in cells such as epithelial or glial cells within the immediate environment of the receptor neurons. These first subcellular localization studies indicate the presence of high levels of NGF in neurons not known to be sensitive to the trophic molecule.
利用相关的光学显微镜和电子显微镜技术，对胚胎小鼠中神经生长因子（NGF）的免疫细胞化学定位进行了研究。在扁平包埋的厚切片中，嗅觉通路的初级感觉神经元因其高水平的NGF样免疫反应性而清晰可见。当在电子显微镜下检查超薄切片时，鼻上皮中存在的HRP反应产物局限于化学感受细胞的细胞质中，包括伸入鼻腔的树突。投射到嗅球的轴突和嗅球内的轴突束也被特异性地染成NGF阳性。反应产物似乎与任何特定的亚细胞结构都无关。柱状支持细胞和基底前体细胞均未显示出任何免疫反应性的证据。在受体神经元紧邻环境中的上皮细胞或神经胶质细胞等细胞中未发现NGF存在的证据。这些首次进行的亚细胞定位研究表明，在已知对这种营养分子不敏感的神经元中存在高水平的NGF。
