Rué G, Bierne J
Laboratoire de Biologie Cellulaire et Générale, Université de Champagne, Reims, France.
Cell Differ Dev. 1988 Sep;25(1):11-22. doi: 10.1016/0922-3371(88)90051-2.
The localization, structure and function of two types of nuclear bodies have been investigated by cytological and cytochemical electron microscopy methods in oocytes from the Hoplonemertean, Amphiporus lactifloreus. Type I nuclear bodies differentiate in contact with the nucleolus-DNA body complex, whereas type II nuclear bodies develop close to the diplotenic chromosomal axes. The structure of type I and type II spherical nuclear bodies, 4-5 micron in width, results from the association of a fibrillar reticulum with some dense included regions. The cytochemical findings following the use of osmium-ammine reaction for DNA and silver reaction for NOR proteins support the hypothesis that type II nuclear bodies, derived from the extranucleolar area, as well as type I nuclear bodies, derived from the nucleolar complex, may be involved in ribosomal biogenesis.
通过细胞学和细胞化学电子显微镜方法，对 Hoplonemertean 的 Amphiporus lactifloreus 卵母细胞中两种核体的定位、结构和功能进行了研究。I 型核体在与核仁 - DNA 体复合体接触时分化，而 II 型核体在双线期染色体轴附近发育。宽度为 4 - 5 微米的 I 型和 II 型球形核体的结构，是由纤维状网络与一些密集的内含区域结合而成。使用锇 - 氨反应检测 DNA 和银反应检测 NOR 蛋白后的细胞化学结果支持了这样的假设，即源自核仁外区域的 II 型核体以及源自核仁复合体的 I 型核体可能参与核糖体生物合成。
