报告 - 金属离子对葡聚糖水解生物催化剂催化效率的作用

REPORT- Role of metal ions on the catalytic efficiency of dextran hydrolyzing biocatalyst.

作者信息

Shahid Faiza, Javed Urooj, Aman Afsheen, Ul Qader Shah Ali

机构信息

Department of Biochemistry, University of Karachi, Karachi, Pakistan.

The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.

出版信息

Pak J Pharm Sci. 2019 Nov;32(6):2761-2764.

PMID:31969313

Abstract

Hydrothermal spring isolate Bacillus megaterium KIBGE-IB31was utilized to produce dextranase. Enzyme was partially purified up to 11.8 fold after dialysis. Different metals ions were tested to explore their behavior with dextranase. It was noticed that cobalt (Co), copper (Cu), magnesium (Mg), manganese (Mn), nickle (Ni) and zinc (Zn) act as activator whilst potassium (K), sodium (Na), barium (Ba), calcium (Ca), mercury (Hg), vanadium (V), aluminum (Al) and ferric (Fe) ions display inhibitory action.

摘要

利用热液泉分离出的巨大芽孢杆菌KIBGE-IB31来生产葡聚糖酶。透析后酶被部分纯化至11.8倍。测试了不同金属离子与葡聚糖酶的相互作用。结果发现钴（Co）、铜（Cu）、镁（Mg）、锰（Mn）、镍（Ni）和锌（Zn）起到激活剂的作用，而钾（K）、钠（Na）、钡（Ba）、钙（Ca）、汞（Hg）、钒（V）、铝（Al）和铁（Fe）离子表现出抑制作用。