Dec Marta, Puchalski Andrzej, Stępień-Pyśniak Dagmara, Marek Agnieszka, Urban-Chmiel Renata
Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
J Vet Med Sci. 2020 Mar 24;82(3):333-336. doi: 10.1292/jvms.19-0533. Epub 2020 Jan 22.
The aim of this study was to determine the susceptibility of Lactobacillus bacteria to selected coccidiostats. Seventy-five Lactobacillus isolates obtained from chickens were classified by MALDI-TOF mass spectrometry and 16S rDNA restriction analysis into seven species, among which L. salivarius (33%) and L. johnsonii (24%) were dominant. Susceptibility of lactobacilli to coccidiostats was determined by broth microdilution method. The ranges of minimum inhibitory concentrations (MICs) were 0.5-≥128 µg/ml for monensin, 0.125-8 µg/ml for salinomycin, ≤0.03-2 µg/ml for lasalocid A, and 4-16 µg/ml for robenidine. Coccidiostats in low concentrations inhibited in vitro growth of most lactobacilli and therefore there is a high probability that administration of this drugs to chickens would reduce the number of lactobacilli in the gut.
本研究的目的是确定乳酸杆菌对选定抗球虫药的敏感性。通过基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF）和16S rDNA限制性分析，将从鸡体内分离得到的75株乳酸杆菌分为7个种，其中唾液乳杆菌（33%）和约翰逊乳杆菌（24%）占主导。采用肉汤微量稀释法测定乳酸杆菌对抗球虫药的敏感性。莫能菌素的最低抑菌浓度（MIC）范围为0.5-≥128µg/ml，盐霉素为0.125-8µg/ml，拉沙洛西A为≤0.03-2µg/ml，氯苯胍为4-16µg/ml。低浓度的抗球虫药在体外抑制了大多数乳酸杆菌的生长，因此给鸡使用这些药物很可能会减少肠道内乳酸杆菌的数量。
