Yang Qianqian, Song Limin, Miao Zhengang, Su Meiling, Liang Wenxing, He Yawen
Key Lab of Integrated Crop Pest Management of Shandong, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, China.
Front Microbiol. 2020 Jan 8;10:2965. doi: 10.3389/fmicb.2019.02965. eCollection 2019.
BcHpt is a core element of the high-osmolarity glycerol (HOG) transduction pathway in . In contrast to other elements of the pathway, which have been characterized and proven to play important roles in vegetative differentiation, fungicide resistance, the multistress response, and virulence in , BcHpt (Histidine-containing phosphotransfer) is essential but uncharacterized in . Our previous study reported the first lysine acetylation site (Lys161) in BcHpt. In this study, the functions of this lysine acetylation site in BcHpt were characterized using site-directed mutagenesis. To mimic Lys161 acetylation, we generated the mutant strain ΔBcHPt + BcHpt-GFP, which exhibited a slower growth rate; lower pathogenicity; higher sensitivity to multiple stresses, including osmotic and oxidative stresses, dicarboximides, and demethylation inhibitors (DMIs); and lower BcSak1 phosphorylation levels than wild-type . Constitutive acetylation of BcHpt Ly161 apparently inhibits hyphal growth, the multistress response, and sensitivity to fungicides in Moreover, the lysine acetylation site affected phosphorylation of the MAPK BcSak1.
BcHpt是[具体物种]中高渗甘油（HOG）转导途径的核心元件。与该途径的其他元件不同，其他元件已被表征并证明在营养分化、抗真菌剂、多重胁迫反应和[具体物种]的毒力中发挥重要作用，而BcHpt（含组氨酸的磷酸转移蛋白）在[具体物种]中是必需的但未被表征。我们之前的研究报道了BcHpt中的第一个赖氨酸乙酰化位点（Lys161）。在本研究中，使用定点诱变对BcHpt中该赖氨酸乙酰化位点的功能进行了表征。为了模拟Lys161乙酰化，我们构建了突变菌株ΔBcHPt + BcHpt-GFP，该菌株表现出较慢的生长速率；较低的致病性；对多种胁迫（包括渗透胁迫和氧化胁迫、二羧酰亚胺和去甲基化抑制剂（DMI））具有更高的敏感性；并且与野生型[具体物种]相比，BcSak1磷酸化水平更低。BcHpt Ly161的组成型乙酰化明显抑制了[具体物种]中的菌丝生长、多重胁迫反应和对杀菌剂的敏感性。此外，赖氨酸乙酰化位点影响了MAPK BcSak1的磷酸化。
