College of Plant Health and Medicine and Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, China.
College of Agriculture, Ludong University, Yantai, 264025, Shandong Province, China.
BMC Genomics. 2022 Feb 18;23(1):145. doi: 10.1186/s12864-022-08372-4.
Lysine 2-hydroxyisobutyrylation (Khib) is a newly discovered posttranslational modification (PTM) and has been identified in several prokaryotic and eukaryotic organisms. Fusarium graminearum, a major pathogen of Fusarium head blight (FHB) in cereal crops, can cause considerable yield loss and produce various mycotoxins that threaten human health. The application of chemical fungicides such as tebuconazole (TEC) remains the major method to control this pathogen. However, the distribution of Khib in F. graminearum and whether Khib is remodified in response to fungicide stress remain unknown.
Here, we carried out a proteome-wide analysis of Khib in F. graminearum, identifying the reshaping of the lysine 2-hydroxyisobutyrylome by tebuconazole, using the most recently developed high-resolution LC-MS/MS technique in combination with high-specific affinity enrichment. Specifically, 3501 Khib sites on 1049 proteins were identified, and 1083 Khib sites on 556 modified proteins normalized to the total protein content were changed significantly after TEC treatment. Bioinformatics analysis showed that Khib proteins are involved in a wide range of biological processes and may be involved in virulence and deoxynivalenol (DON) production, as well as sterol biosynthesis, in F. graminearum.
Here, we provided a wealth of resources for further study of the roles of Khib in the fungicide resistance of F. graminearum. The results enhanced our understanding of this PTM in filamentous ascomycete fungi and provided insight into the remodification of Khib sites during azole fungicide challenge in F. graminearum.
赖氨酸 2-羟基异丁酰化（Khib）是一种新发现的翻译后修饰（PTM），已在几种原核和真核生物中被鉴定。禾谷镰刀菌是谷物赤霉病（FHB）的主要病原体，可导致大量减产，并产生各种威胁人类健康的真菌毒素。化学杀菌剂如戊唑醇（TEC）的应用仍然是控制该病原体的主要方法。然而，Khib 在禾谷镰刀菌中的分布以及 Khib 是否响应杀菌剂胁迫而发生重塑尚不清楚。
在这里，我们通过最先进的高分辨率 LC-MS/MS 技术与高特异性亲和富集相结合，对禾谷镰刀菌中的 Khib 进行了全蛋白质组分析，鉴定了戊唑醇对赖氨酸 2-羟基异丁酰化组的重塑。具体而言，在 TEC 处理后，在 1049 种蛋白质上鉴定出 3501 个 Khib 位点，在 556 个经修饰的蛋白质上鉴定出 1083 个 Khib 位点，其变化与总蛋白质含量归一化后差异显著。生物信息学分析表明，Khib 蛋白参与广泛的生物学过程，可能与禾谷镰刀菌的毒力和脱氧雪腐镰刀菌烯醇（DON）产生以及甾醇生物合成有关。
在这里，我们为进一步研究 Khib 在禾谷镰刀菌杀菌剂抗性中的作用提供了丰富的资源。这些结果增强了我们对丝状子囊菌中这种 PTM 的理解，并为唑类杀菌剂挑战期间 Khib 位点的重塑提供了新的见解。
