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λ噬菌体的ea22基因：尽管序列多样性高，但原噬菌体功能得以保留。

The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity.

作者信息

Dydecka Aleksandra, Bloch Sylwia, Necel Agnieszka, Topka Gracja, Węgrzyn Alicja, Tong Jinge, Donaldson Logan W, Węgrzyn Grzegorz, Nejman-Faleńczyk Bożena

机构信息

Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.

Laboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822, Gdańsk, Poland.

出版信息

Virus Genes. 2020 Apr;56(2):266-277. doi: 10.1007/s11262-020-01734-8. Epub 2020 Jan 22.

DOI:10.1007/s11262-020-01734-8
PMID:31970620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7093339/
Abstract

The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein.

摘要

λ样噬菌体的exo-xis区域包含一些开放阅读框和基因，这些基因似乎在进化上具有重要意义。然而，到目前为止该区域很少受到关注。在本研究中，我们提供了证据表明，与该区域其他已鉴定的基因（包括ea8.5、orf61、orf60a和orf63）不同，该区域最大的基因ea22更倾向于溶原途径而非裂解途径。我们的实验还表明Ea22与噬菌体整合酶蛋白（Int）之间存在一些功能上的相似性。虽然Ea22在λ噬菌体和stx噬菌体中表现相似并不奇怪，但我们也观察到了一些差异，这些差异可能源于每种蛋白质羧基末端相当大的序列差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/0a3f80b48f8a/11262_2020_1734_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/dacceb95510e/11262_2020_1734_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/7ca53917dc7d/11262_2020_1734_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/4e41a1b1e5cb/11262_2020_1734_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/b1ae9bc27088/11262_2020_1734_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/0a3f80b48f8a/11262_2020_1734_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/dacceb95510e/11262_2020_1734_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/7ca53917dc7d/11262_2020_1734_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/4e41a1b1e5cb/11262_2020_1734_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/b1ae9bc27088/11262_2020_1734_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38a9/7093339/0a3f80b48f8a/11262_2020_1734_Fig5_HTML.jpg

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