以及2024年从重症监护病房患者中分离出的菌株：噬菌体来源的一般特征、原噬菌体、解聚酶和酯酶
and Isolates Obtained from Intensive Care Unit Patients in 2024: General Characterization, Prophages, Depolymerases and Esterases of Phage Origin.
作者信息
Kolupaeva Nadezhda V, Kolupaeva Lyubov V, Evseev Peter V, Skryabin Yuriy P, Lazareva Elena B, Chernenkaya Tatyana V, Volozhantsev Nikolay V, Popova Anastasia V
机构信息
State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia.
Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
出版信息
Viruses. 2025 Apr 26;17(5):623. doi: 10.3390/v17050623.
and are significant nosocomial pathogens worldwide. In this study, the general characterization of and isolates obtained from the blood of intensive care unit patients of the multidisciplinary scientific and practical center of emergency medicine from January to September 2024 was performed. Prophage regions and prophage-derived tailspike polysaccharide-depolymerizing or -modifying enzymes within these isolates were identified and characterized in detail using a refined workflow. The protocol, encompassing a comprehensive survey of all predicted bacterial proteins, revealed an average of 6.0 prophage regions per genome, including regions putatively derived from filamentous phages, and 4.8 prophage regions per isolate. Analysis of these putative prophage regions indicated that most were related to previously isolated, yet unclassified, temperate phages infecting and . However, certain identified sequences likely originated from phages representing novel groups comparatively distant from known phages.
[具体病原菌名称1]和[具体病原菌名称2]是全球范围内重要的医院感染病原菌。在本研究中，对2024年1月至9月从多学科科学与实践急诊医学中心重症监护病房患者血液中分离得到的[具体病原菌名称1]和[具体病原菌名称2]菌株进行了一般特征分析。使用优化的工作流程对这些菌株中的前噬菌体区域以及前噬菌体衍生的尾刺多糖解聚或修饰酶进行了详细鉴定和表征。该方案涵盖了对所有预测的细菌蛋白的全面调查，结果显示每个[具体病原菌名称1]基因组平均有6.0个前噬菌体区域，包括可能源自丝状噬菌体的区域，每个[具体病原菌名称2]菌株平均有4.8个前噬菌体区域。对这些假定的前噬菌体区域的分析表明，大多数与先前分离但未分类的感染[具体病原菌名称1]和[具体病原菌名称2]的温和噬菌体有关。然而，某些鉴定出的序列可能源自与已知噬菌体相对较远的新型噬菌体群体。
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