Teixeira M L, Haddad A
Departamento de Morfologia, Faculdade de Medicina, Ribeirão Preto-SP, Brasil.
Cell Tissue Res. 1988 Oct;254(1):209-16. doi: 10.1007/BF00220036.
Two-month-old female Swiss mice that had come into estrus were injected intravenously with L-3H-fucose and killed at 5, 15, 40 min, and 4 h after injection. Pieces of the isthmus and of the ampulla of the uterine tubes were processed for light- and electron-microscopic radioautography. Incorporation of 3H-fucose was more intense in the isthmian secretory cells than in the ciliated cells of the ampulla. Electron-microscopic radioautography of the isthmian secretory cells demonstrated that 3H-fucose was incorporated into newly synthesized glycoproteins in the Golgi apparatus from where labelled glycoproteins migrated mainly to secretory granules and apical microvilli. The histochemical technique using ruthenium red confirmed the presence of glycoproteins in the contents of the secretory granules released to the lumen of the uterine tubes as demonstrated by radioautography. Other glycoproteins are transported inside small vesicles and most likely are related to the renewal of the plasma membrane. The role of the secretory glycoproteins in various events of mammalian reproduction is discussed.
对进入发情期的2月龄雌性瑞士小鼠静脉注射L-3H-岩藻糖，并在注射后5分钟、15分钟、40分钟和4小时处死。对输卵管峡部和壶腹部组织切片进行光镜和电镜放射自显影处理。峡部分泌细胞中3H-岩藻糖的掺入比壶腹纤毛细胞更强烈。峡部分泌细胞的电镜放射自显影显示，3H-岩藻糖掺入高尔基体中新合成的糖蛋白中，标记的糖蛋白主要从高尔基体迁移到分泌颗粒和顶端微绒毛。使用钌红的组织化学技术证实了放射自显影所示释放到输卵管管腔的分泌颗粒内容物中存在糖蛋白。其他糖蛋白通过小泡运输，很可能与质膜更新有关。本文讨论了分泌糖蛋白在哺乳动物生殖各种事件中的作用。
