Kopriwa B M, Levine G M, Nadler N J
Histochemistry. 1984;80(6):519-22. doi: 10.1007/BF02400965.
Half distance values for electron microscopic (EM) radioautographs with the isotopes 3H and 125I were determined using Ilford L4 emulsion processed with either fine grain, solution physical development, or filamentous grain, chemical development with D-19b. 3H- and 125I-line sources, obtained by cutting perpendicular sections from sections of 3H-labeled methacrylate or 125I-labeled thyroid glands, were processed for EM radioautography. The distribution of silver grains around a line source was determined by measuring their distance from the source in photographs of EM radioautographs. The number of silver grains per unit distance from the line source was plotted on graphs and half distance values were calculated. With solution physical development, the half distance value was 74 nm for 3H and 80 nm for 125I; whereas with D-19 b development it was 187 nm for 3H and 157 nm for 125I. Since solution physical development produced a reduction of about 50% in the half distance values for both isotopes, it is concluded that the production of fine grain by this method provides better resolution for EM radioautography than filamentous grain development with D-19b.
使用伊尔福L4乳剂，通过细颗粒、溶液物理显影或丝状颗粒、用D - 19b进行化学显影，测定了用同位素³H和¹²⁵I制作的电子显微镜（EM）放射自显影片的半距离值。通过从³H标记的甲基丙烯酸酯切片或¹²⁵I标记的甲状腺切片上切取垂直切片获得³H和¹²⁵I线源，并对其进行EM放射自显影处理。通过在EM放射自显影片的照片中测量银颗粒与线源的距离，确定线源周围银颗粒的分布。将距线源每单位距离的银颗粒数量绘制在图表上，并计算半距离值。采用溶液物理显影时，³H的半距离值为74纳米，¹²⁵I为80纳米；而采用D - 19b显影时，³H为187纳米，¹²⁵I为157纳米。由于溶液物理显影使两种同位素的半距离值均降低了约50%，因此得出结论，与用D - 19b进行丝状颗粒显影相比，这种方法产生的细颗粒为EM放射自显影提供了更好的分辨率。
