Department of Gastroenterology, The First People's Hospital of Fuyang, Hangzhou, Zhejiang, China.
J Biol Regul Homeost Agents. 2019 Nov-Dec;33(6):1675-1683. doi: 10.23812/19-184-A.
The purpose of this paper was to investigate the dynamic trend of SIRT5 (Sirtuins 5) protein expression in gastric cancer cells, for which a hypoxic gastric cancer cell line was established. Afterward, the parental gastric cancer cell group and hypo group were designed, and the levels of related proteins and mRNAs were detected by using Western blot along with real-time quantitative fluorescence PCR (RT-PCR). The results showed that the expression of SIRT5 in hypoxic gastric cancer cells increased significantly, which could increase the phosphorylation level of c-MET (hepatocyte growth factor receptor) and promote the migration of gastric cancer cells. When the expression of SIRT5 protein was observed under hypoxic conditions, SIRT5 silencing significantly reduced the migration ability of MGC803/hypo cells. It could be predicted that SIRT5-mediated protein desuccinylation played an important role in promoting the migration of hypoxic gastric cancer cells. Therefore, the migration rate of gastric cancer cells could be affected by controlling the expression of SIRT5 protein, which provides a novel idea for the treatment of gastric cancer in the future.
本文旨在研究缺氧状态下胃癌细胞中 SIRT5（沉默信息调节因子 2 相关酶 5）蛋白表达的动态变化趋势，为此建立了缺氧胃癌细胞系。随后设计了亲本胃癌细胞组和缺氧组，并通过 Western blot 和实时荧光定量聚合酶链式反应（RT-PCR）检测相关蛋白和 mRNAs 的水平。结果表明，缺氧胃癌细胞中 SIRT5 的表达显著增加，这可能会增加 c-MET（肝细胞生长因子受体）的磷酸化水平并促进胃癌细胞的迁移。当观察缺氧条件下 SIRT5 蛋白的表达时，SIRT5 沉默显著降低了 MGC803/hypo 细胞的迁移能力。可以预测，SIRT5 介导的蛋白质去琥珀酰化在促进缺氧胃癌细胞迁移中发挥重要作用。因此，通过控制 SIRT5 蛋白的表达可以影响胃癌细胞的迁移率，这为未来胃癌的治疗提供了新的思路。
