Gu Wen, Qian Qinyi, Xu Yinkai, Xu Xiaolan, Zhang Liping, He Songbing, Li Dechun
Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Ultrasonography, Changshu No. 2 People's Hospital, Changshu, China.
J Int Med Res. 2021 Feb;49(2):300060520986355. doi: 10.1177/0300060520986355.
Accumulating evidence illustrates that sirtuins (SIRTs) regulate autophagy and apoptosis in cancer cells; however, the role of SIRT5 in gastric cancer (GC) cells remains unknown. In this study, we examined the role of SIRT5 in GC cells.
We detected SIRT5 protein levels in freshly collected samples from patients with GC. Next, we studied the function of SIRT5 in autophagy. Furthermore, the signaling pathway through which SIRT5 enhanced autophagy in GC cells was detected. In addition, we established a GC cell apoptosis model to analyze the role of SIRT5 in apoptosis.
SIRT5 expression was downregulated in GC tissues. We discovered that SIRT5 promoted autophagy in GC cells. We demonstrated that SIRT5 enhanced autophagy in GC cells the AMP-activated protein kinase-mammalian target of rapamycin signaling pathway. In addition, SIRT5 was degraded during apoptosis in GC cells. Meanwhile, we observed that calpains and caspase-related proteins were associated with SIRT5-related GC cell apoptosis.
SIRT5 is a crucial regulator of autophagy and apoptosis in GC cell lines that can maintain the balance of autophagy and apoptosis.
越来越多的证据表明，沉默调节蛋白（SIRTs）在癌细胞中调节自噬和凋亡；然而，SIRT5在胃癌（GC）细胞中的作用仍不清楚。在本研究中，我们检测了SIRT5在GC细胞中的作用。
我们检测了GC患者新鲜采集样本中的SIRT5蛋白水平。接下来，我们研究了SIRT5在自噬中的功能。此外，还检测了SIRT5增强GC细胞自噬的信号通路。另外，我们建立了GC细胞凋亡模型来分析SIRT5在凋亡中的作用。
GC组织中SIRT5表达下调。我们发现SIRT5促进GC细胞的自噬。我们证明SIRT5通过AMP激活的蛋白激酶-雷帕霉素哺乳动物靶标信号通路增强GC细胞的自噬。此外，SIRT5在GC细胞凋亡过程中被降解。同时，我们观察到钙蛋白酶和半胱天冬酶相关蛋白与SIRT5相关的GC细胞凋亡有关。
SIRT5是GC细胞系自噬和凋亡的关键调节因子，可维持自噬和凋亡的平衡。
