Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng, 224007, PR China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, PR China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng, 224007, PR China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China.
Fish Shellfish Immunol. 2020 Mar;98:318-323. doi: 10.1016/j.fsi.2020.01.033. Epub 2020 Jan 20.
Procambarus clarkii is one of the most important aquatic invertebrates in China and has high commercial value. However, aquaculture has suffered great economic loss due to outbreaks of infectious diseases in P. clarkii. To identify red swamp crayfish related proteins involved in the response to bacterial infection, we analysed immune-related proteins following lipopolysaccharide (LPS) stimulation by quantitative proteomics. The proteome of the hepatopancreas of P. clarkii challenged with LPS and phosphate-buffered saline was analysed to evaluate the immune response. Based on liquid chromatography coupled with tandem mass spectrometry, 16 upregulated and 29 downregulated proteins were identified. A Gene Ontology analysis demonstrated 5 biological process, 11 cellular component, and 6 molecular function subcategories. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the identified proteins were mainly involved in metabolism, phagosome, and ribosome. Real-time quantitative reverse transcription-PCR revealed that eight immune-related genes were upregulated after LPS stimulation compared to the control. Taken together, the data enhance our understanding of the immune response of crayfish to LPS.
克氏原螯虾是中国最重要的水生无脊椎动物之一，具有很高的商业价值。然而，由于克氏原螯虾爆发传染病，水产养殖遭受了巨大的经济损失。为了鉴定参与细菌感染反应的红螯螯虾相关蛋白，我们通过定量蛋白质组学分析了脂多糖（LPS）刺激后的免疫相关蛋白。通过分析用 LPS 和磷酸盐缓冲盐水处理的克氏原螯虾的肝胰腺蛋白质组，评估免疫反应。基于液相色谱-串联质谱联用技术，鉴定出 16 个上调和 29 个下调蛋白。GO 分析显示 5 个生物学过程、11 个细胞组分和 6 个分子功能亚类。京都基因与基因组百科全书通路富集分析表明，鉴定出的蛋白主要参与代谢、吞噬体和核糖体。实时定量 RT-PCR 显示，与对照组相比，LPS 刺激后 8 个免疫相关基因上调。总之，这些数据增强了我们对螯虾对 LPS 免疫反应的理解。
