School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, PR China.
Department of Zoology and Fisheries, University of Agriculture, Faisalabad 38000, Pakistan.
Fish Shellfish Immunol. 2017 Dec;71:144-150. doi: 10.1016/j.fsi.2017.10.010. Epub 2017 Oct 7.
Crustacean hepatopancreas regulates metabolic processes, biogenesis and innate immune processes, and the knowledge on its immune genes are crucial to understand antimicrobial mechanisms. In this study, we reported the transcriptomic profile of Procambarus clarkii hepatopancreas after poly I:C administration using high-throughput sequencing. Following de novo assembly 56,716 unigene sequences with an average length of 810 bp was obtained. The unigene sequences were annotated to three ontologies including cellular components, biological processes and molecular functions, further 56,716 unigene sequences were mapped to 25 COG categories. A total of 2497 differentially expressed genes (DEGs) were identified following the comparative analysis between poly I:C treated and control group, and then KEGG enrichment analysis were performed to detect immune related pathways. Quantitative real time polymerase chain reaction showed that the selected DEGs significantly up-regulated following poly I:C administration in comparison to control group. The transcriptomic sequence information will improve the knowledge of this economically important crustacean, and will shed light on its antiviral immune mechanisms.
甲壳动物的肝胰腺调节代谢过程、生物发生和先天免疫过程，了解其免疫基因对于理解抗菌机制至关重要。在这项研究中，我们使用高通量测序报告了聚肌胞苷酸处理后克氏原螯虾肝胰腺的转录组图谱。通过从头组装，获得了 56716 条平均长度为 810bp 的 unigene 序列。unigene 序列被注释到三个本体论中，包括细胞成分、生物过程和分子功能，进一步将 56716 条 unigene 序列映射到 25 个 COG 类别。通过对聚肌胞苷酸处理组和对照组之间的比较分析，共鉴定出 2497 个差异表达基因（DEGs），然后进行了 KEGG 富集分析以检测免疫相关途径。定量实时聚合酶链反应显示，与对照组相比，聚肌胞苷酸处理后所选 DEGs 显著上调。转录组序列信息将提高这种具有经济重要性的甲壳动物的知识水平，并揭示其抗病毒免疫机制。
