Chen Yujun, Gong Tao, Yu Cilong, Qian Xiang, Wang Xiaohao
Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen 518060, China.
Micromachines (Basel). 2020 Jan 21;11(2):120. doi: 10.3390/mi11020120.
Simplifying tedious sample preparation procedures to improve analysis efficiency is a major challenge in contemporary analytical chemistry. Solid phase microextraction (SPME), a technology developed for rapid sample pretreatment, has flexibility in design, geometry, and calibration strategies, which makes it a useful tool in a variety of fields, especially environmental and life sciences. Therefore, it is important to study the coupling between the microfluidic electrospray ionization (ESI) chip integrated with the solid phase microextraction (SPME) module and the electrospray mass spectrometer (MS). In our previous work, we designed a solid phase microextraction (SPME) module on a microfluidic chip through geometric design. However, automation and calibration methods for the extraction process remain unresolved in the SPME on-chip domain, which will lead to faster and more accurate results. This paper discusses the necessity to design a micromixer structure that can produce different elution conditions on the microfluidic chip. By calculating the channel resistances, the microfluidic chip's integrated module with the micromixer, SPME, and ESI emitters optimize the geometry structure. We propose the annular channel for SPME to perform the resistances balance of the entire chip. Finally, for SPME on a single chip, this work provides a quantitation calibration method to describe the distribution of the analytes between the sample and the extraction phase before reaching the adsorption equilibrium.
简化繁琐的样品制备程序以提高分析效率是当代分析化学中的一项重大挑战。固相微萃取（SPME）是一种为快速样品预处理而开发的技术，在设计、几何形状和校准策略方面具有灵活性，这使其成为各种领域，特别是环境和生命科学领域中的有用工具。因此，研究集成有固相微萃取（SPME）模块的微流控电喷雾电离（ESI）芯片与电喷雾质谱仪（MS）之间的耦合非常重要。在我们之前的工作中，我们通过几何设计在微流控芯片上设计了一个固相微萃取（SPME）模块。然而，片上固相微萃取领域中萃取过程的自动化和校准方法仍未得到解决，而这将带来更快、更准确的结果。本文讨论了在微流控芯片上设计一种能够产生不同洗脱条件的微混合器结构的必要性。通过计算通道电阻，对集成有微混合器、固相微萃取和电喷雾发射器的微流控芯片的模块进行几何结构优化。我们提出了用于固相微萃取的环形通道，以实现整个芯片的电阻平衡。最后，对于单芯片上的固相微萃取，这项工作提供了一种定量校准方法，用于描述在达到吸附平衡之前分析物在样品和萃取相之间的分布情况。
