乙醇对大鼠肝细胞脂肪酸代谢的影响与营养状况的相关性。

Dependence with nutritional status of the ethanol effects on fatty acid metabolism in rat hepatocytes.

Maquedano A, Guzmán M, Castro J

Departamento de Bioquímica, Facultad de Química, Universidad Complutense, Madrid, Spain.

Int J Biochem. 1988;20(9):937-41. doi: 10.1016/0020-711x(88)90178-4.

Abstract

The effects of ethanol on fatty acid synthesis, esterification and oxidation were studied in hepatocytes isolated from fed and 24 hr fasted rats. 2. [3H]H2O was preferentially incorporated into the glycerol backbone of triglycerides and phospholipids. Addition of ethanol markedly increased the incorporation of this label in both classes of glycerolipids; the increase was higher in fasted rat hepatocytes, both in the glycerol backbone and acyl groups of glycerolipids. 3. Ethanol increased [U-14C]palmitate incorporation into triglycerides only in hepatocytes from fasted rats. 4. [14C]CO2 and total acid soluble product formation from [1-14C]palmitate resulted inhibited by ethanol both in the fed and the fasted state.

摘要

研究了乙醇对从喂食和禁食24小时的大鼠分离出的肝细胞中脂肪酸合成、酯化和氧化的影响。2. [3H]H2O优先掺入甘油三酯和磷脂的甘油主链中。添加乙醇显著增加了这两种甘油脂质中该标记物的掺入；在禁食大鼠肝细胞中，甘油脂质的甘油主链和酰基中的增加更高。3. 乙醇仅在禁食大鼠的肝细胞中增加了[U-14C]棕榈酸掺入甘油三酯。4. 在喂食和禁食状态下，乙醇均抑制了[1-14C]棕榈酸产生的[14C]CO2和总酸溶性产物的形成。