cf. （甲藻纲）分子系统发育、形态学以及巴西分离株和野外样品中海生毒素的检测。

cf. (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil.

机构信息

Laboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, Brazil.

Laboratório de Biodiversidade e Evolução Molecular, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 512, Rio de Janeiro, RJ 22290-240, Brazil.

出版信息

Toxins (Basel). 2020 Jan 22;12(2):70. doi: 10.3390/toxins12020070.

DOI:10.3390/toxins12020070

PMID:31979144

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7076807/

Abstract

Recurrent blooms of cf. have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1-D3 and D8-D10 regions of the LSU rDNA and ITS loci, and the morphology of . cf. isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy. Morphometric analysis of different strains and field populations from diverse locations were compared. Molecular analysis showed that six of the seven sequences grouped at the large "Atlantic/Mediterranean/Pacific" sub-clade, while one sequence branched in a sister clade with sequences from Madeira Island and Greece. The toxin profile of strains and bloom field samples from Rio de Janeiro were dominated by OVTX-a and -b, with total cell quotas (31.3 and 39.3 pg cell) in the range of that previously reported for strains of . cf. .

摘要

cf. 藻的反复大量繁殖已在巴西和地中海被报道，伴随而来的还有生态学和后者的健康影响。本研究基于 LSU rDNA 的 D1-D3 和 D8-D10 区以及 ITS 基因座、cf. 藻分离株和现场种群的形态学，提供了来自巴西热带和亚热带沿海地区以及三个大洋岛屿的分子数据。此外，还提供了来自热带海岸的三个单细胞的 ITS 序列。对来自里约热内卢的两个克隆培养物和两个野外水华的细胞中所含的 PLTX 及其类似物、OVTXs 的毒素特征和数量进行了研究。使用明场和荧光显微镜观察了形态。比较了来自不同地点的不同菌株和现场种群的形态计量分析。分子分析表明，7 个序列中的 6 个序列聚在大的“大西洋/地中海/太平洋”亚群中，而一个序列与来自马德拉岛和希腊的序列形成姐妹群。来自里约热内卢的菌株和水华野外样本的毒素图谱主要由 OVTX-a 和 -b 组成，细胞总含量（31.3 和 39.3 pg 细胞）与之前报道的 cf. 藻菌株的含量相当。