Ide H, Aono H
Biological Institute, Tohoku University, Sendai, Japan.
Dev Biol. 1988 Dec;130(2):767-73. doi: 10.1016/0012-1606(88)90365-x.
Distal and proximal mesoderm of chick limb bud was respectively dissociated and cultured in the medium containing various concentrations of retinoic acid (RA). At low concentrations (5-50 ng/ml), RA promoted proliferation and chondrogenesis in the distal mesodermal cells. The distal cells of stage 20-24 limb buds were responsive to RA, although those of stages 25-27 were unresponsive. Both the cells of anterior and posterior regions of the distal mesoderm were responsive to RA, while the cells of proximal mesoderm were unresponsive. At higher concentrations, the growth-promoting effect of RA was reduced and chondrogenesis in the distal cells was rather inhibited. These results were discussed in relation to the role of RA as the morphogen in normal limb development and experimental duplicate formation.
将鸡胚肢芽的远端和近端中胚层分别解离，并在含有不同浓度视黄酸（RA）的培养基中培养。在低浓度（5-50 ng/ml）时，RA促进远端中胚层细胞的增殖和软骨形成。20-24期肢芽的远端细胞对RA有反应，而25-27期的细胞则无反应。远端中胚层前后区域的细胞均对RA有反应，而近端中胚层细胞无反应。在较高浓度时，RA的促生长作用减弱，远端细胞的软骨形成受到抑制。结合RA作为正常肢体发育和实验性重复形成中的形态发生原的作用，对这些结果进行了讨论。
